{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T03:35:04Z","timestamp":1769052904389,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T00:00:00Z","timestamp":1369785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004799","name":"Australian Dental Research Foundation (ADRF)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004799","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Dental Board of New South Wales, Australia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Previously we demonstrated uniformly strong expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24 in the epithelial attachment to the tooth and in the migrating epithelium of the periodontitis lesion. Titers of serum antibodies autoreactive with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24 peptide correlated with reduced severity of periodontal disease. Ligation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24 expressed by oral epithelial cells induced formation of tight junctions that limited paracellular diffusion. In this study, we aimed to reveal that the lack of uniform expression of tight junction components in the pocket epithelium of periodontitis lesions is likely to contribute to increased paracellular permeability to bacterial products. This is proposed as a potential driver of the immunopathology of periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>An epithelial culture model with close correspondence for expression patterns for tight junction components in periodontal epithelia was used. Immunohistochemical staining and confocal laser scanning microscopy were used to analyse patterns of expression of gingival epithelial tight junction components.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The minimally inflamed gingival attachment was characterized by uniformly strong staining at cell contacts for the tight junction components zona occludens\u20101, zona occludens\u20102, occludin, junction adhesion molecule\u2010A, claudin\u20104 and claudin\u201015. In contrast, the pocket epithelium of the periodontal lesion showed scattered, uneven staining for these components. This pattern correlated closely with that of unstimulated oral epithelial cells in culture. Following ligation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24 expressed by these cells, the pattern of tight junction component expression of the minimally inflamed gingival attachment developed rapidly.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>There was evidence for non\u2010uniform and focal expression only of tight junction components in the pocket epithelium. In the cell\u2010culture model, ligation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24 induced a tight junction expression profile equivalent to that observed for the minimally inflamed gingival attachment. Ligation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24 expressed by gingival epithelial cells by lectin\u2010like receptors of commensal oral streptococci could mediate the phenotype of health, whereas pathogenic organisms associated with periodontal disease might not signal effectively through <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>24.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12102","type":"journal-article","created":{"date-parts":[[2013,5,29]],"date-time":"2013-05-29T08:06:46Z","timestamp":1369814806000},"page":"253-259","source":"Crossref","is-referenced-by-count":18,"title":["Expression patterns of tight junction components induced by <scp>CD<\/scp>24 in an oral epithelial cell\u2010culture model correlated to affected periodontal tissues"],"prefix":"10.1111","volume":"49","author":[{"given":"P.","family":"Ye","sequence":"first","affiliation":[{"name":"Institute of Dental Research Westmead Millennium Institute Westmead Hospital Westmead Australia"},{"name":"Westmead Centre for Oral Health Westmead Hospital Westmead Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yu","sequence":"additional","affiliation":[{"name":"Microscopy Laboratory Westmead Millennium Institute Westmead Hospital Westmead Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Simonian","sequence":"additional","affiliation":[{"name":"Institute of Dental Research Westmead Millennium Institute Westmead Hospital Westmead Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Institute of Dental Research Westmead Millennium Institute Westmead Hospital Westmead Australia"},{"name":"Faculty of Dentistry The University of Sydney Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400102"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113167"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.22.010305.104219"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00558.2003"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-8-235"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.9.2287"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00930"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01235"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-186"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/15419060600726209"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01811-05"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-008-0418-7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.043"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH673>3.0.CO;2-T"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2001.300308.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2005.02692.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.147.4.1412"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2010.70"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1168988"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-10-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.07.067"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711600313"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.08.033"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01666871"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2010.07.010"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.123.6.1777"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2005.01.008"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-008-0424-9"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00581.2004"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00189578"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1998.tb00320.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2012.11.004"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12102","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12102","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:42:54Z","timestamp":1696207374000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,29]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1111\/jre.12102"],"URL":"https:\/\/doi.org\/10.1111\/jre.12102","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,29]]}}}