{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:27:20Z","timestamp":1791268040131,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T00:00:00Z","timestamp":1373500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NH&MRC","award":["627143"],"award-info":[{"award-number":["627143"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>The complex microenvironment of the periodontal wound creates many challenges associated with multitissue regeneration of periodontal lesions. Recent characterization of mesenchymal stem cell\u2010like populations residing in periodontal ligament tissues has shown that these cells exhibit features of postnatal stem cells. Despite these advances, a lack of consistency in design of preclinical studies and a limited study of allogeneic transplantation applications has restricted our understanding of their clinical utility in the treatment of periodontal disease. The aim of this study was to assess the regenerative potential of allogeneic periodontal ligament stem cells (PDLSCs) in a rat periodontal fenestration defect mode and to identify an optimal end time\u2010point suitable for quantitative assessment of tissue regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Periodontal fenestration defects, created in Sprague Dawley rats, were treated with allogeneic <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s seeded onto Gelfoam<jats:sup>\u00ae<\/jats:sup> (Absorbable gelatin sponge; Pharmacia Corporation, Kalamazoo, <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> USA<\/jats:styled-content>) or with Gelfoam<jats:sup>\u00ae<\/jats:sup> alone, or remained untreated. Experimental rats were killed at 7, 14, 21 or 28\u00a0d after surgery and the tissues were processed for immunohistochemical and histomorphometric examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Defects treated with <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s showed significantly greater percentage bone fill and length of new bone bridge compared with the untreated group or the group treated withGelfoam<jats:sup>\u00ae<\/jats:sup> alone on days 14 and 21. Similarly, a statistically significant difference was achieved within specimens retrieved on day 21 for analysis of regeneration of cementum\/periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>)\u2010like structures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The present investigation shows that allogeneic <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s have a marked ability to repair periodontal defects by forming bone, <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> and cementum\u2010like tissue <jats:italic>in vivo<\/jats:italic>. The results suggest that treatment periods of 14 and 21\u00a0d are optimal end time\u2010points for quantitative assessment of periodontal regeneration within the rodent fenestration\u2010defect model utilized in the present study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12111","type":"journal-article","created":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T07:20:37Z","timestamp":1373527237000},"page":"333-345","source":"Crossref","is-referenced-by-count":78,"title":["Assessment of the regenerative potential of allogeneic periodontal ligament stem cells in a rodent periodontal defect model"],"prefix":"10.1111","volume":"49","author":[{"given":"J.","family":"Han","sequence":"first","affiliation":[{"name":"Colgate Australian Clinical Dental Research Centre School of Dentistry University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Menicanin","sequence":"additional","affiliation":[{"name":"Colgate Australian Clinical Dental Research Centre School of Dentistry University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Marino","sequence":"additional","affiliation":[{"name":"Colgate Australian Clinical Dental Research Centre School of Dentistry University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Stomatology Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Mrozik","sequence":"additional","affiliation":[{"name":"Colgate Australian Clinical Dental Research Centre School of Dentistry University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Gronthos","sequence":"additional","affiliation":[{"name":"Mesenchymal Stem Cell Laboratory School of Medical Sciences University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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