{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T21:00:50Z","timestamp":1774558850419,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T00:00:00Z","timestamp":1375747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH\/NIDCR","award":["DE019872"],"award-info":[{"award-number":["DE019872"]}]},{"name":"Talentia Scholarship Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>In the chronic established periodontal lesion, the proliferation and migration potential of periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>) cells are significantly compromised. Thus, the progressive loss of tissue integrity is favored and normal healing and regeneration compromised. Periostin, a known <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> marker, modulates cell\u2013matrix interactions, cell behavior, as well as the matrix biomechanics and <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> homeostasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate whether periostin restores the regenerative potential of <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> cells in terms of proliferation, migration, and activation of survival signaling pathways after being challenged by <jats:italic><jats:styled-content style=\"fixed-case\">Porphyromonas<\/jats:styled-content> gingivalis<\/jats:italic> lipopolysaccharides and tumor <jats:styled-content style=\"fixed-case\">n<\/jats:styled-content>ecrosis <jats:styled-content style=\"fixed-case\">f<\/jats:styled-content>actor <jats:styled-content style=\"fixed-case\">a<\/jats:styled-content>lpha \u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human PDL (hPDL) cells were cultured under different conditions: control, periostin (50 or 100\u00a0ng\/mL), and fibroblast growth factor 2 (10\u00a0ng\/mL) to evaluate cell proliferation (by Ki67), cell migration (by scratch assays) and PI3K\/AKT\/mTOR pathway activation (by western blot analyses of total AKT, phospho\u2010AKT and PS6). A different set of cultures was challenged by adding tumor necrosis factor alpha \u03b1 (10\u00a0ng\/mL) and <jats:italic>P.\u00a0gingivalis<\/jats:italic> lipopolysaccharides (200\u00a0ng\/mL) to evaluate the effects of periostin as described above.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Periostin significantly increased cell proliferation (twofold), migration (especially at earlier time points and low dose) and activation of survival signaling pathway (higher phosphorylation of <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content>6). Furthermore, periostin promoted similar cellular effects even after being challenged with proinflammatory cytokines and bacterial virulence factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Periostin acts as an important modulator of hPDL cell\u2013matrix dynamics. It modulates h<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> proliferation, migration and <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>3<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\/m<jats:styled-content style=\"fixed-case\">TOR<\/jats:styled-content> pathway. It also helps in overcoming the altered biological phenotype that chronic exposure to periodontal pathogens and proinflammatory cytokines produce in h<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12120","type":"journal-article","created":{"date-parts":[[2013,8,6]],"date-time":"2013-08-06T21:14:48Z","timestamp":1375823688000},"page":"405-414","source":"Crossref","is-referenced-by-count":50,"title":["Periostin increases migration and proliferation of human periodontal ligament fibroblasts challenged by tumor necrosis factor \u2010\u03b1 and <i>Porphyromonas <\/i><i>gingivalis <\/i><scp>lipopolysaccharides<\/scp>"],"prefix":"10.1111","volume":"49","author":[{"given":"M.","family":"Padial\u2010Molina","sequence":"first","affiliation":[{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan Ann Arbor MI USA"},{"name":"Department of Oral Surgery and Implant Dentistry School of Dentistry University of Granada Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. L.","family":"Volk","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan Ann Arbor MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. F.","family":"Rios","sequence":"additional","affiliation":[{"name":"Department of Periodontics and Oral Medicine School of Dentistry University of Michigan Ann Arbor MI USA"},{"name":"Michigan Center for Oral Health Research School of Dentistry University of Michigan Ann Arbor MI 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