{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:49:16Z","timestamp":1791312556172,"version":"4.3.1"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T00:00:00Z","timestamp":1395705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"COECYTJal-UdeG","award":["06-2009-990"],"award-info":[{"award-number":["06-2009-990"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Periodontal disease is a chronic bacterial infection characterized by connective tissue breakdown and alveolar bone destruction because of inflammatory and immune response caused by periodontopathogens and long\u2010term release of reactive oxygen species. A high number of reactive oxygen species result in periodontal tissue damage through multiple mechanisms such as lipid peroxidation, protein denaturation and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage. The aim of this study was to evaluate <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and oxidative damage in subjects with chronic or aggressive periodontitis and healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Buccal mucosa cells and whole saliva were collected from 160 subjects, who were divided into three groups: subjects with chronic periodontitis (CP) (<jats:italic>n<\/jats:italic>\u00a0=\u00a058), subjects with aggressive periodontitis (AgP) (<jats:italic>n<\/jats:italic>\u00a0=\u00a042) and a control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a060). <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage was determined by counting micronuclei (<jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>) and nuclear abnormalities (<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>s) in exfoliated cells, including binucleated cells, cells with nuclear buds and karyolitic, karyorrhectic, condensed chromatin and pyknotic cells. The degree of oxidative stress was determined by quantifying 8\u2010hydroxy\u20102\u2032\u2010deoxyguanosine (8\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>dG) in whole saliva.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Subjects with <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> or AgP presented significantly more (\u00a0<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>s and higher levels of 8\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>dG (\u00a0<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) compared with the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that subjects with periodontitis (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> or AgP) exhibited an increase in the frequency of <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> NA<\/jats:styled-content>s and 8\u2010<jats:styled-content style=\"fixed-case\">OH<\/jats:styled-content>dG, which is directly related to <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage. In addition, a positive correlation exists between oxidative stress produced by periodontitis disease and <jats:styled-content style=\"fixed-case\">MN<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12175","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T12:07:13Z","timestamp":1395749233000},"page":"28-36","source":"Crossref","is-referenced-by-count":45,"title":["Increased micronuclei and nuclear abnormalities in buccal mucosa and oxidative damage in saliva from patients with chronic and aggressive periodontal diseases"],"prefix":"10.1111","volume":"50","author":[{"given":"A. L.","family":"Zamora\u2010Perez","sequence":"first","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Odontolog\u00eda Centro Universitario de Ciencias de la Salud Universidad de Guadalajara Guadalajara Jalisco M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. M.","family":"Ortiz\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Odontolog\u00eda Centro Universitario de Ciencias de la Salud Universidad de Guadalajara Guadalajara Jalisco M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. P.","family":"Lazalde\u2010Ramos","sequence":"additional","affiliation":[{"name":"Unidad Acad\u00e9mica de Ciencias Qu\u00edmicas Universidad Aut\u00f3noma de Zacatecas Zacatecas M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Guerrero\u2010Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Odontolog\u00eda Centro Universitario de Ciencias de la Salud Universidad de Guadalajara Guadalajara Jalisco M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. 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