{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:36:38Z","timestamp":1790026598194,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,14]],"date-time":"2014-07-14T00:00:00Z","timestamp":1405296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Institute of Research Management and Monitoring","award":["PG101-2012B"],"award-info":[{"award-number":["PG101-2012B"]}]},{"name":"University Malaya"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>A number of species\/phylotypes have been newly implicated as putative periopathogens. The objective of this study was to explore associations among classical and new pathogens in subgingival biofilm and to assess their relative importance to chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Pooled subgingival biofilm samples were obtained from 40 patients with chronic periodontitis and 40 healthy controls. Taqman q\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assays were used to determine the absolute and relative counts of <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>orphyromonas gingivalis<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>annerella forsythia<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>reponema denticola<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>arvimonas micra<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>ilifactor alocis<\/jats:italic>, oral Synergistetes and oral <jats:styled-content style=\"fixed-case\">TM<\/jats:styled-content>7s. Microbial associations were assessed using cluster analysis. Different statistical models were used to explore associations between microbial parameters and periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median log and relative counts were lowest for <jats:styled-content style=\"fixed-case\">TM<\/jats:styled-content>7s (4.4 and 0.0016%, respectively) and highest for oral Synergistetes (7.2 and 1.4%, respectively). Oral Synergistetes clustered strongly with the red complex, particularly <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0forsythia<\/jats:italic> (100% rescaled similarity). All species\/phylotypes except <jats:styled-content style=\"fixed-case\">TM<\/jats:styled-content>7s were significantly associated with periodontitis (<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ann\u2013<jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>hitney test; <jats:italic>p<\/jats:italic>\u00a0\u2264\u00a00.005). However, <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0gingivalis<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>.\u00a0alocis<\/jats:italic> lost association after adjusting for confounders (ordinal regression). In receiving operator characteristic curve analysis, the log counts of oral Synergistetes were the best markers of periodontitis (82.5% sensitivity and specificity), followed by those of <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0forsythia<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0micra<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>.\u00a0denticola<\/jats:italic>. In prediction analysis, however, <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0micra<\/jats:italic> was the only microbial predictor of periodontal parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Oral Synergistetes are presented here as new members of the red complex, with relative importance to periodontitis exceeding that of the classical members. <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>.\u00a0micra<\/jats:italic> is shown as an important periodontal pathogen warranting more attention.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12210","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T17:19:44Z","timestamp":1405444784000},"page":"320-329","source":"Crossref","is-referenced-by-count":37,"title":["Quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case\u2013control study"],"prefix":"10.1111","volume":"50","author":[{"given":"N. 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