{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T17:24:10Z","timestamp":1781717050728,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T00:00:00Z","timestamp":1405468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education and Science, Republic of Serbia","award":["#41008"],"award-info":[{"award-number":["#41008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Gingival recession is defined as soft and hard tissue displacement resulting in root surface exposure. The optimal outcome of gingival recession treatment is complete, predictable and long\u2010lasting root coverage with a significant level of tissue regeneration. Tissue engineering, which applies active regeneration principles, presents the contemporary treatment approach in the restitution and regeneration of lost tissues. The objective of the present study was to evaluate and compare the clinical results of application of an autologous fibroblast cell culture (<jats:styled-content style=\"fixed-case\">AFCC<\/jats:styled-content>) on a collagen matrix and a connective tissue graft (<jats:styled-content style=\"fixed-case\">CTG<\/jats:styled-content>) placed under a coronally advanced flap (<jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>), in the treatment of single and multiple gingival recessions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Eighteen patients from the Department of Periodontology, School of Dentistry, University of Belgrade, were randomly enrolled in this study. Inclusion criteria were the bilateral presence of Miller Class I or <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> single or multiple maxillary gingival recessions. A split\u2010mouth design was used in the study. The experimental group was treated with <jats:styled-content style=\"fixed-case\">AFCC<\/jats:styled-content> on a collagen scaffold, which was placed under a <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>. The control group received a combination of <jats:styled-content style=\"fixed-case\">CTG<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CAF<\/jats:styled-content>. Clinical parameters such as gingival recession coverage, keratinized tissue width, clinical attachment level and gingival index were recorded at baseline and at 12\u00a0mo postoperatively. The oral hygiene level was assessed by plaque index evaluation. Postoperative healing was evaluated through the healing index, recorded 1, 2 and 3\u00a0wk postoperatively. The final esthetic outcome was assessed using the mean root coverage esthetic score (<jats:styled-content style=\"fixed-case\">RES<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Statistically significant improvement of all parameters assessed was found compared with baseline. A statistically significant difference between groups was observed only in keratinized tissue width. Greater keratinized tissue width is still obtained with the use of <jats:styled-content style=\"fixed-case\">CTG<\/jats:styled-content>. Regarding the tissue\u2010healing results, no statistically significant difference was achieved. The <jats:styled-content style=\"fixed-case\">RES<\/jats:styled-content> results were similar for both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the limitations of the present study, both procedures proved to be efficient in gingival recession treatment. <jats:styled-content style=\"fixed-case\">AFCC<\/jats:styled-content>, as a novel tissue\u2010engineering concept and living cell\u2010based therapy, proved to be a reliable and successful treatment concept.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12215","type":"journal-article","created":{"date-parts":[[2014,7,16]],"date-time":"2014-07-16T07:07:42Z","timestamp":1405494462000},"page":"363-370","source":"Crossref","is-referenced-by-count":32,"title":["Clinical application of autologous fibroblast cell culture in gingival recession treatment"],"prefix":"10.1111","volume":"50","author":[{"given":"I.","family":"Milinkovic","sequence":"first","affiliation":[{"name":"Department of Periodontology Belgrade School of Dentistry Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Aleksic","sequence":"additional","affiliation":[{"name":"Department of Periodontology Belgrade School of Dentistry Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Jankovic","sequence":"additional","affiliation":[{"name":"Department of Periodontology Belgrade School of Dentistry Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Popovic","sequence":"additional","affiliation":[{"name":"Institute of Virology, Vaccines and Sera \u201cTorlak\u201d Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bajic","sequence":"additional","affiliation":[{"name":"Department of Periodontology Belgrade School of Dentistry Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Cakic","sequence":"additional","affiliation":[{"name":"Department of Periodontology Belgrade School of Dentistry Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Lekovic","sequence":"additional","affiliation":[{"name":"Department of Periodontology Belgrade School of Dentistry Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,16]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Clinical Periodontology and Implant Dentistry","author":"Lindhe J","year":"2003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.110382"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2003.8.1.303"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.29.s3.11.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.11607\/prd.1337"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.8.1126"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110373"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050390"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100671"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100604"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.6.867"},{"key":"e_1_2_7_13_1","first-page":"552","article-title":"Tissue engineering technology for gingival augmentation procedures: a case report","volume":"20","author":"Pini\u2010Prato GP","year":"2000","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.2.262"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2002.73.7.748"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.080017"},{"key":"e_1_2_7_17_1","first-page":"14","article-title":"Root coverage using the free soft tissue autograft following citric acid application. 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