{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T18:04:14Z","timestamp":1780423454068,"version":"3.54.1"},"reference-count":66,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T00:00:00Z","timestamp":1410220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Natural Science Foundation Projects","award":["81170995"],"award-info":[{"award-number":["81170995"]}]},{"name":"National 973 Projects","award":["2012CB619100"],"award-info":[{"award-number":["2012CB619100"]}]},{"name":"State Key Clinical Specialty of Implant Dentistry Project"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study was to investigate the effects of osteoprotegerin (<jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>) gene therapy on alveolar bone resorption caused by experimental periodontitis in rats, thus forming a foundation for potential clinical applications of <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content> gene therapy in the treatment of periodontitis and peri\u2010implantitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>To study the effects of OPG on alveolar bone protection, an experimental periodontitis model was used by placing a bacterial plaque retentive silk ligature in the gingival sulcus around the maxillary second molar tooth, injection of <jats:italic>Porphyromonas gingivalis<\/jats:italic> and high carbohydrate diet. A total of 30 Sprague\u2013Dawley rats were randomly divided into three groups, with 10 rats in each group: group I (control) was treated with 10\u00a0\u03bcL normal saline injection; group II with 10\u00a0\u03bcL mock vector; and group III with 10\u00a0\u03bcL local OPG gene transfer by transfection with <jats:italic>in vitro<\/jats:italic> constructed pcDNA3.1\u2010<jats:styled-content style=\"fixed-case\">human OPG (pcDNA3.1\u2010hOPG)<\/jats:styled-content>. A subperiosteal injection was done adjacent to the second molars on days 0, 7, 14 and 21. Four weeks later, all animals were killed and radiographic, histological and immunohistochemical examinations were performed. Statistical analysis included ANOVA and LSD\u2010Bonferroni test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Group <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content> gene therapy) had significantly enhanced (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) integrated optical density of <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content>, had significantly decreased alveolar bone resorption volume and active osteoclast number (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) through descriptive histological examination when compared with the other two groups at week 4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Local recombinant <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content> plasmid\u2010mediated gene therapy suppresses osteoclastogenesis <jats:italic>in vivo<\/jats:italic> and inhibits alveolar bone height reduction caused by experimental periodontitis in rats. <jats:styled-content style=\"fixed-case\">OPG<\/jats:styled-content> gene therapy may be beneficial in preventing progressive periodontal bone loss.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12224","type":"journal-article","created":{"date-parts":[[2014,9,9]],"date-time":"2014-09-09T12:36:24Z","timestamp":1410266184000},"page":"434-443","source":"Crossref","is-referenced-by-count":17,"title":["<i>In vivo<\/i> osteoprotegerin gene therapy preventing bone loss induced by periodontitis"],"prefix":"10.1111","volume":"50","author":[{"given":"H.","family":"Tang","sequence":"first","affiliation":[{"name":"West China Hospital of Stomatology Sichuan University Chengdu China"},{"name":"The Prince Philip Dental Hospital The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Mattheos","sequence":"additional","affiliation":[{"name":"The Prince Philip Dental 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