{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:27:20Z","timestamp":1773286040348,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T00:00:00Z","timestamp":1418860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"KAKENHI","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"KIBAN C","award":["24593116"],"award-info":[{"award-number":["24593116"]}]},{"name":"KIBAN C","award":["22592325"],"award-info":[{"award-number":["22592325"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Oxytalan fibers are categorized as a microfibril assembly without elastin deposition, and are unique components in the periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>). However, little is known about their formation during <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> development. To clarify the mechanisms of oxytalan fiber formation in developing <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>, we performed immunohistochemical analysis to detect the direct expression of fibrillin\u20101 and fibrillin\u20102, which are major components of microfibrils.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Frozen sections of lower molars from mice at several stages of growth were prepared without chemical fixation and decalcification using the film transfer method. Immunostaining was performed with anti\u2010fibrillin\u20101 and \u20102, and anticytokeratin antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fibrillin\u20101 was not expressed in the dental follicle during the crown forming stage. At postneonatal day 9, fibrillin\u20101 expression started with meshwork appearance between the epithelial cells from <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>ertwig's epithelial root sheath at the root dentin surface. Fibirillin\u20102 was detected much earlier than fibrillin\u20101 expression. Fibrillin\u20102 was expressed with a liner appearance, running parallel to the root axis in <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>, and was partially co\u2010expressed with cytokeratin 14 expression in <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>ertwig's epithelial root sheath. Furthermore, we detected both fibrillin\u20101 and fibrillin\u20102 expression in human <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>. Fibrillin\u20101 was detected in fibers with a vertically oriented root axis in <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>. Fibrillin\u20102 was widely expressed in <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>, including around the epithelial cell rests of Malassez. Fibrillin\u20101 and fibrillin\u20102 were clearly co\u2010expressed in thick fiber structures in human <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest that both fibrillin\u20101 and fibrillin\u20102 expression is required to form thick oxytalan fibers in <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>. Based on the expression patterns for fibrillin\u20101 and fibrillin\u20102, they have different functions during tooth root and <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> development. Early expression of fibrillin\u20102 may regulate dental epithelial cell behavior during root and <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> development.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12256","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T08:57:46Z","timestamp":1418979466000},"page":"714-720","source":"Crossref","is-referenced-by-count":9,"title":["Immunohistochemical expression of fibrillin\u20101 and\u00a0fibrillin\u20102 during tooth development"],"prefix":"10.1111","volume":"50","author":[{"given":"M.","family":"Kira\u2010Tatsuoka","sequence":"first","affiliation":[{"name":"Section of Pediatric Dentistry Department of\u00a0Oral Growth and Development Fukuoka Dental College Fukuoka Japan"},{"name":"Section\u00a0of Functional Structure Department\u00a0of\u00a0Morphological Biology Fukuoka\u00a0Dental College Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Oka","sequence":"additional","affiliation":[{"name":"Section of Pediatric Dentistry Department of\u00a0Oral Growth and Development Fukuoka Dental College Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Tsuruga","sequence":"additional","affiliation":[{"name":"Section\u00a0of Functional Structure Department\u00a0of\u00a0Morphological Biology Fukuoka\u00a0Dental College Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ozaki","sequence":"additional","affiliation":[{"name":"Section of Pediatric Dentistry Department of\u00a0Oral Growth and Development Fukuoka Dental College Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Sawa","sequence":"additional","affiliation":[{"name":"Section\u00a0of Functional Structure Department\u00a0of\u00a0Morphological Biology Fukuoka\u00a0Dental 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