{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T03:06:40Z","timestamp":1774926400626,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T00:00:00Z","timestamp":1425513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>The angiotensin type 1 (AT1) receptor has been implicated in the pathogenesis of inflammatory bone disorders. This study aimed to investigate the effect of an AT1 receptor antagonist in infection\u2010induced and arthritis\u2010associated alveolar bone loss in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Mice were subjected to <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> oral infection or antigen\u2010induced arthritis and treated daily with 10\u00a0mg\/kg of the prototype AT1 antagonist, losartan. Treatment was conducted for 30\u00a0d in the infectious condition and for 17\u00a0d and 11\u00a0d in the preventive or therapeutic regimens in the arthritic model, respectively. The mice were then killed, and the maxillae, serum and knee joints were collected for histomorphometric and immunoenzymatic assays. <jats:italic>In vitro<\/jats:italic> osteoclast assays were performed using RAW 264.7 cells stimulated with <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> lipopolysacharide (LPS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Arthritis and <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> infection triggered significant alveolar bone loss in mice and increased the levels of myeloperoxidase and of TRAP<jats:sup>+<\/jats:sup> osteoclasts in periodontal tissues. Losartan abolished such a phenotype, as well as the arthritis joint inflammation. Both arthritis and <jats:italic>A.\u00a0actinomycetemcomitans<\/jats:italic> conditions were associated with the release of tumor necrosis factor alpha (TNF\u2010\u03b1), interferon\u2010gamma, interleukin\u201017 and chemokine (C\u2010X\u2010C motif) ligand 1 and an increased RANKL\/osteoprotegerin ratio in periodontal tissues, but such expression decreased after losartan treatment, except for TNF\u2010\u03b1. The therapeutic approach was as beneficial as the preventive one. <jats:italic>In vitro<\/jats:italic>, losartan prevented LPS\u2010induced osteoclast differentiation and activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The blockade of AT1 receptor exerts anti\u2010inflammatory and anti\u2010osteoclastic effects, thus protecting periodontal tissues in distinct pathophysiological conditions of alveolar bone loss.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12269","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T11:26:12Z","timestamp":1425554772000},"page":"814-823","source":"Crossref","is-referenced-by-count":18,"title":["Protective effects of the angiotensin type 1 receptor antagonist losartan in infection\u2010induced and arthritis\u2010associated alveolar bone loss"],"prefix":"10.1111","volume":"50","author":[{"given":"C. M.","family":"Queiroz\u2010Junior","sequence":"first","affiliation":[{"name":"Department of Oral Surgery and Pathology Faculdade de Odontologia Universidade Federal de Minas Gerais Minas Gerais Brazil"},{"name":"Department of Biochemistry and Immunology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. D.","family":"Silveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. R.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology Faculdade de Odontologia Universidade Federal de Minas Gerais Minas Gerais Brazil"},{"name":"Department of Biochemistry and Immunology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P.","family":"Moura","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology Faculdade de Odontologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. F. M.","family":"Madeira","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology Faculdade de Odontologia Universidade Federal de Minas Gerais Minas Gerais Brazil"},{"name":"Department of Microbiology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. M.","family":"Soriani","sequence":"additional","affiliation":[{"name":"Department of General Biology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Morphology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. Y.","family":"Fukada","sequence":"additional","affiliation":[{"name":"Department of Physics and Chemistry* Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Microbiology Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. A.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology Faculdade de Odontologia Universidade Federal de Minas Gerais Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2012-202199"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3669"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510376402"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510385236"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01671.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199804)175:1<89::AID-JCP10>3.0.CO;2-J"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22335"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-098954"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.080264"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00041552-200105000-00005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.081006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2013.05.012"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12160"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-013-0886-8"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00886-12"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.09.005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01046-13"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101195"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.07.022"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807610200"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2012.01886.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1520005"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/648537"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.9.5577"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000148824.93600.F3"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.1.66"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/846052"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2008.01.010"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/327417"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/35046102"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-010116"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10041"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1999.tb00161.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1997.tb00535.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI30074"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2297"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12269","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12269","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T11:48:39Z","timestamp":1696160919000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,5]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/jre.12269"],"URL":"https:\/\/doi.org\/10.1111\/jre.12269","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,5]]}}}