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Periodontal measurements included probing depth, clinical attachment level, visible supragingival biofilm and bleeding on probing. Subgingival biofilm samples were collected from periodontal sites (50% with <jats:styled-content style=\"fixed-case\">probing depth <\/jats:styled-content>\u2264\u00a04\u00a0mm and 50% with <jats:styled-content style=\"fixed-case\">probing depth <\/jats:styled-content>\u2265\u00a05\u00a0mm) and whole\u2010genomic\u2010amplified <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> was obtained. The <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> samples were subjected to amplification of a 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene fragment using universal bacterial primers, followed by <jats:styled-content style=\"fixed-case\">DGGE<\/jats:styled-content> analysis of the amplified gene sequences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The non\u2010<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected group presented higher mean full\u2010mouth visible supragingival biofilm (p\u00a0=\u00a00.004), <jats:styled-content style=\"fixed-case\">bleeding on probing<\/jats:styled-content> (p\u00a0=\u00a00.006), <jats:styled-content style=\"fixed-case\">probing depth<\/jats:styled-content> (p\u00a0&lt;\u00a00.001) and <jats:styled-content style=\"fixed-case\">clinical attachment level<\/jats:styled-content> (p\u00a0=\u00a00.001) in comparison with the <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected group. <jats:styled-content style=\"fixed-case\">DGGE<\/jats:styled-content> analysis revealed 81 distinct bands from all 33 individuals. Banding profiles revealed a higher diversity of the bacterial communities in the subgingival biofilm of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients with chronic periodontitis. Moreover, cluster and principal component analyses demonstrated that the bacterial community profiles differed between these two conditions. High interindividual and intra\u2010individual variability in banding profiles were observed for both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected patients with chronic periodontitis present greater subgingival microbial diversity. In addition, the bacterial communities associated\u00a0with <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected and non\u2010<jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected individuals are different in structure.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12287","type":"journal-article","created":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T02:04:46Z","timestamp":1433383486000},"page":"95-102","source":"Crossref","is-referenced-by-count":14,"title":["Subgingival bacterial community profiles in <scp>HIV<\/scp>\u2010infected Brazilian adults with chronic periodontitis"],"prefix":"10.1111","volume":"51","author":[{"given":"D. C.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Department of Endodontics and Molecular Microbiology Laboratory Est\u00e1cio de S\u00e1 University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. S.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Department of Endodontics and Molecular Microbiology Laboratory Est\u00e1cio de S\u00e1 University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"J. F.","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Department of Endodontics and Molecular Microbiology Laboratory Est\u00e1cio de S\u00e1 University Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. L.","family":"Carmo","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Prof. Paulo de G\u00f3es Federal University of Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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