{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:50:07Z","timestamp":1779904207811,"version":"3.53.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T00:00:00Z","timestamp":1433980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["20102ZLNJ5"],"award-info":[{"award-number":["20102ZLNJ5"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>In the present study, the early stages of <jats:italic>in\u00a0vitro<\/jats:italic> bone formation in collagenated porcine scaffolds cultured with human periodontal ligament cells were investigated. The comparison between the osteogenic potential of this structure in basal and differentiating culture media was explored to predict the mechanism of its biological behavior as graft in human defect. Results were validated by synchrotron radiation X\u2010Ray phase contrast computed microtomography (micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>). As the periodontal disease plays a key role in systemic and oral diseases, it is crucial to find advanced therapeutic clinical interventions to repair periodontal defects. This has been recently explored using cells and tissues developed <jats:italic>in\u00a0vitro<\/jats:italic> that should ideally be immunologically, functionally, structurally and mechanically identical to the native tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p><jats:italic>In vitro<\/jats:italic> cultures of human <jats:styled-content style=\"fixed-case\">periodontal ligament cell<\/jats:styled-content>s, easily obtained by scraping of alveolar crestal and horizontal fibers of the periodontal ligament, were seeded on to collagenated porcine blocks constituted by natural cancellous and cortical bone. 3D images were obtained by <jats:styled-content style=\"fixed-case\">synchrotron radiation<\/jats:styled-content> micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> and processed with a phase\u2010retrieval algorithm based on the transport of intensity equation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Starting from the second week of culture, newly formed mineralized bone was detected in all the scaffolds, both in basal and differentiating media. Bone mineralization was proved to occur preferentially in the trabecular portion and in differentiating media.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The chosen method, supported by phase contrast micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> analysis, successfully and quantitatively monitored the early stages of bone formation and the rate of the bioscaffold resorption in basal and differentiating culture media.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12289","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T17:21:41Z","timestamp":1434734501000},"page":"112-124","source":"Crossref","is-referenced-by-count":51,"title":["Osteogenic potential of dualblocks cultured with human periodontal ligament stem cells: <i>in vitro<\/i> and synchrotron microtomography 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