{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:27:35Z","timestamp":1788992855464,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T00:00:00Z","timestamp":1433203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1057835"],"award-info":[{"award-number":["APP1057835"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Histone deacetylase inhibitors (<jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>i) are being considered to treat chronic inflammatory diseases at low doses. Currently <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>i that are more specific are being developed to target particular <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>s; therefore, this study aimed to determine levels and distribution of class I and <jats:styled-content style=\"fixed-case\">II HDAC<\/jats:styled-content> in human gingival samples obtained from patients with chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Gingival biopsies were obtained from patients with and without (mild inflammation, no bone loss) periodontitis. Total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was isolated for real\u2010time quantitative polymerase chain reaction to determine expression of <jats:styled-content style=\"fixed-case\">HDACs<\/jats:styled-content> 1\u201310. Immunohistochemistry was used to determine protein distribution of <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>s 1, 5, 8 and 9. Factor <jats:styled-content style=\"fixed-case\">VIII<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CD<\/jats:styled-content>3 and tartrate resistant acid phosphatase (TRAP) were detected in serial sections to identify blood vessels, lymphocytes, pre\u2010osteoclasts and osteoclasts cells respectively. Tumour necrosis factor \u03b1 (TNF\u2010\u03b1) expression was also assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> 1, 5, 8 and 9 were significantly upregulated in\u00a0chronic periodontitis gingival tissues compared to non\u2010periodontitis samples\u00a0(<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Significantly higher <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> 1 protein expression was observed in chronic periodontitis samples (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and was associated with\u00a0<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3, TRAP and TNF\u2010\u03b1\u2010positive cells. <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> 1, 5, 8 and 9 were expressed strongly by the factor <jats:styled-content style=\"fixed-case\">VIII<\/jats:styled-content>\u2010positive microvasculature in the chronic periodontitis gingival tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> 1, 5, 8 and 9 expression was higher in gingival tissues from patients with chronic periodontitis compared to non\u2010periodontitis samples. Results suggest that these <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>s could therefore be targeted with specific acting <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>i.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12290","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T23:53:48Z","timestamp":1433289228000},"page":"143-151","source":"Crossref","is-referenced-by-count":29,"title":["Class I and II histone deacetylase expression in human chronic periodontitis gingival tissue"],"prefix":"10.1111","volume":"51","author":[{"given":"M. D.","family":"Cantley","sequence":"first","affiliation":[{"name":"Discipline of Anatomy and Pathology School of Medical Sciences University of Adelaide Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A. S. S. 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