{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:25:04Z","timestamp":1773307504438,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T00:00:00Z","timestamp":1439164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Bio & Medical Technology Development Program of the National Research Foundation"},{"name":"Korean government","award":["NRF-2013M34A9B2076480"],"award-info":[{"award-number":["NRF-2013M34A9B2076480"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2016,6]]},"abstract":"Background and Objective<\/jats:title>The predictability of conventional periodontal treatments for damaged periodontal tissue is limited, particularly on the regeneration of new cementum. As signaling molecules, a range of growth factors has been used to promote periodontal regeneration on periodontal ligament (PDL<\/jats:styled-content>) and cementum defects. A preameloblast\u2010conditioned medium (PA<\/jats:styled-content>\u2010CM<\/jats:styled-content>) was prepared from cultured murine apical bud cells, which can differentiate into ameloblasts. We examined the effect of PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> on PDL<\/jats:styled-content> cells and cementoblasts in\u00a0vitro<\/jats:italic> and evaluated histologically the effects of PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> on the regeneration of experimentally induced periodontal defects in\u00a0vivo<\/jats:italic>.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>In vitro,<\/jats:italic> the effects of PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> on the migration of human PDL<\/jats:styled-content> cells were examined using a scratch wound healing assay and a transwell assay. The differentiation and mineralization potential of PA<\/jats:styled-content>\u2010CM<\/jats:styled-content>\u2010treated human PDL<\/jats:styled-content> cells and murine cementoblastic OCCM<\/jats:styled-content>\u201030 cells was examined by real\u2010time polymerase chain reaction and Alizarin red\u2010S staining. In vivo<\/jats:italic>, six mongrel dogs (12\u201316\u00a0kg; 6\u20138\u00a0mo old) were used. Twenty\u2010four roots were replanted with either, (i) only periodontal defects (n\u00a0<\/jats:italic>=\u00a0<\/jats:italic>12; control group), or (ii) periodontal defects and PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> treatment (n\u00a0<\/jats:italic>=\u00a0<\/jats:italic>12; experimental group). In the experimental group, the PDL<\/jats:styled-content> and cementum between notches was removed using a Gracey curette and soaked in 0.08\u00a0mL<\/jats:styled-content> water containing 80\u00a0\u03bcg of a PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> for 2\u00a0min. The dogs were killed at 4 and 8\u00a0wk post\u2010surgery.<\/jats:p><\/jats:sec>Results<\/jats:title>The in\u00a0vitro<\/jats:italic> results showed that PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> stimulated the migration of PDL<\/jats:styled-content> cells and promoted the differentiation and mineralization of PDL<\/jats:styled-content> cells and cementoblasts. Real\u2010time polymerase chain reaction analysis revealed stronger expression of Runx2<\/jats:italic>, Osx<\/jats:italic>, OC<\/jats:styled-content><\/jats:italic>, Bsp<\/jats:italic> and Cap <\/jats:italic>mRNA<\/jats:styled-content>s in the PA<\/jats:styled-content>\u2010CM<\/jats:styled-content>\u2010treated PDL<\/jats:styled-content> cells and cementoblasts than those in the control cells. In vivo<\/jats:italic>, newly formed PDL<\/jats:styled-content>\u2010like tissue and cementum\u2010like tissue were observed partially between the root surfaces and newly formed bone in the experimental group. The regenerated PDL<\/jats:styled-content>\u2010like tissue in the experimental group was significantly higher than that in the control group at 8\u00a0wk (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.05). The replacement resorption on the experimental group was significantly lower than that in the control group at 8\u00a0wk (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.05). In addition, the amount of newly formed cementum\u2010like tissue in the experimental group was significantly higher than that in the control group at 4 and 8\u00a0wk (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.05).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These results suggest that PA<\/jats:styled-content>\u2010CM<\/jats:styled-content> has the potential to regenerate periodontal tissues in PDL<\/jats:styled-content> and cementum defects.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12307","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T06:21:07Z","timestamp":1439187667000},"page":"284-294","source":"Crossref","is-referenced-by-count":6,"title":["Periodontal healing with a preameloblast\u2010conditioned medium in dogs"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8818-549X","authenticated-orcid":false,"given":"S.\u2010J.","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Periodontology School of Dentistry Chosun University Gwangju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.\u2010S.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oral Histology\u2010Developmental Biology and Dental Research Institute School of Dentistry Seoul National University Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.\u2010O.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dentistry Chosun University Gwangju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.\u2010H.","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Periodontology Research Institute for Periodontal Regeneration College of Dentistry Yonsei University Seoul South 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