{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T02:56:58Z","timestamp":1772593018503,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T00:00:00Z","timestamp":1447027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Perforated barrier membranes open channels between the suprabony and intrabony compartments of the defect, which could allow for more physiologic cellular interactions between different components of the periodontium during guided tissue regeneration surgery. To test this assumption, this study was designed to evaluate levels of vascular endothelial cell growth factor (<jats:styled-content style=\"fixed-case\">VEGF)<\/jats:styled-content> and platelet\u2010derived growth factor (<jats:styled-content style=\"fixed-case\">PDGF)<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BB<\/jats:styled-content> in gingival crevicular fluid during the early stages of healing of localized intrabony defects treated with perforated membranes (PMs) or non\u2010PMs, as compared with open flap debridement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Thirty non\u2010smoking patients with severe chronic periodontitis participated in this prospective, randomized and single blinded trial. Each patient contributed one interproximal defect that was randomly assigned to the <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content> group (<jats:italic>n<\/jats:italic> = 10), occlusive membrane (<jats:styled-content style=\"fixed-case\">OM<\/jats:styled-content>) group (<jats:italic>n<\/jats:italic> = 10) or open flap debridement (<jats:styled-content style=\"fixed-case\">OFD<\/jats:styled-content>) group (<jats:italic>n<\/jats:italic> = 10). Plaque index, gingival index, probing depth, clinical attachment level and the intrabony depth of the defect were measured at baseline and reassessed at 6 and 9 mo after therapy. Gingival crevicular fluid samples were collected on days 1, 3, 7, 14, 21 and 30 d after therapy for the changes in <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BB<\/jats:styled-content> levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>During the early stages of healing (1, 3 and 7 d), the mean <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BB<\/jats:styled-content> concentrations at sites treated with <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">OFD<\/jats:styled-content> peaked with a statistically significant difference as compared with the <jats:styled-content style=\"fixed-case\">OM<\/jats:styled-content>\u2010treated group. <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PDGF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">BB<\/jats:styled-content> levels at sites treated with <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">OFD<\/jats:styled-content> were not statistically different. Growth factor levels decreased sharply in the samples obtained at days 21 and 30 with non\u2010significant differences between the three groups. Nine months after therapy, the <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content>\u2010treated group showed a statistically significant improvement in probing depth, clinical attachment level and intrabony defect compared to the <jats:styled-content style=\"fixed-case\">OM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">OFD<\/jats:styled-content> groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the limits of the present study, one can conclude that <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content> coverage of periodontal defects is associated with initial gingival crevicular fluid growth factor upregulation that could improve the clinical outcomes of guided tissue regeneration surgery.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12321","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T11:54:45Z","timestamp":1447070085000},"page":"407-416","source":"Crossref","is-referenced-by-count":20,"title":["Gingival crevicular fluid vascular endothelial cell growth factor and platelet\u2010derived growth factor\u2010<scp>BB<\/scp> release profile following the use of perforated barrier membranes during treatment of intrabony defects: a randomized clinical trial"],"prefix":"10.1111","volume":"51","author":[{"given":"A. 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