{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:11:47Z","timestamp":1791900707387,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T00:00:00Z","timestamp":1444262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Helsinki University Hospital Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Background and Objective<\/jats:title>The objective of this study was to evaluate the levels of MMP<\/jats:styled-content>\u20108, MMP\u20109, TIMP<\/jats:styled-content>\u20101 and interleukin\u20101beta (IL<\/jats:styled-content>\u20101\u03b2) in gingival crevicular fluid during the early and late stages of healing in gingival recession sites treated with coronally advanced flap plus platelet\u2010rich fibrin (CAF<\/jats:styled-content>+PRF<\/jats:styled-content>) compared with CAF<\/jats:styled-content> plus connective tissue graft (CAF<\/jats:styled-content>+CTG<\/jats:styled-content>).<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>Twenty\u2010four nonsmoking patients with Miller Class I or Class II<\/jats:styled-content> localized gingival recession defects in bilateral sites received treatment with either CAF<\/jats:styled-content>+PRF<\/jats:styled-content> (PRF<\/jats:styled-content> group) or CAF<\/jats:styled-content>+CTG<\/jats:styled-content> (CTG<\/jats:styled-content> group). Gingival crevicular fluid samples were collected at baseline and at 10\u00a0d and 1\u00a0mo, 3\u00a0mo and 6\u00a0mo after surgery. The levels of MMP<\/jats:styled-content>\u20108, MMP\u20109, TIMP<\/jats:styled-content>\u20101 and IL<\/jats:styled-content>\u20101\u03b2 in gingival crevicular fluid were measured using a time\u2010resolved immunofluorometric assay and ELISAs.<\/jats:p><\/jats:sec>Results<\/jats:title>Gingival crevicular fluid levels of IL<\/jats:styled-content>\u20101\u03b2 were significantly elevated in the CTG<\/jats:styled-content> group at 10\u00a0d compared with baseline (p<\/jats:italic>\u00a0<\u00a00.05). At 10\u00a0d after surgery, the levels of TIMP<\/jats:styled-content>\u20101 in gingival crevicular fluid showed a significant decrease in the CTG<\/jats:styled-content> group compared with the PRF<\/jats:styled-content> group (p<\/jats:italic>\u00a0<\u00a00.05). The levels of IL<\/jats:styled-content>\u20101\u03b2 and MMP<\/jats:styled-content>\u20108 in gingival crevicular fluid were significantly lower in the PRF<\/jats:styled-content> group than in the CTG<\/jats:styled-content> group at 10\u00a0d (p<\/jats:italic>\u00a0<\u00a00.05). No significant differences were found in all clinical and biochemical parameters at 1, 3, and 6\u00a0mo between study groups (p<\/jats:italic>\u00a0>\u00a00.05).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Root coverage with CAF<\/jats:styled-content>+PRF<\/jats:styled-content> has a significant effect on increasing gingival crevicular fluid TIMP<\/jats:styled-content>\u20101 levels and suppressing gingival crevicular fluid MMP<\/jats:styled-content>\u20108 and IL<\/jats:styled-content>\u2010\u03b2 levels at 10\u00a0d. Gingival crevicular fluid levels of MMP<\/jats:styled-content>\u20108, MMP\u20109, TIMP<\/jats:styled-content>\u20101 and IL<\/jats:styled-content>\u20101\u03b2 did not seem to be affected by the technique at later phases of wound healing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12325","type":"journal-article","created":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T07:07:14Z","timestamp":1444288034000},"page":"481-488","source":"Crossref","is-referenced-by-count":25,"title":["Cytokine (interleukin\u20101beta) and MMP levels in gingival crevicular fluid after use of platelet\u2010rich fibrin or connective tissue graft in the treatment of localized gingival recessions"],"prefix":"10.1111","volume":"51","author":[{"given":"G.","family":"Eren","sequence":"first","affiliation":[{"name":"Department of Periodontology School of Dentistry Ege University Bornova\u2010\u0130zmir 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