{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T17:36:36Z","timestamp":1779384996919,"version":"3.53.1"},"reference-count":59,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T00:00:00Z","timestamp":1447027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Although overexpression of the nuclear factor \u03baB inhibitory and ubiquitin\u2010editing enzyme A20 is thought to be involved in the pathogenesis of inflammatory diseases, its function in periodontal disease remains unknown. The aims of the present study were to evaluate A20 expression in patients with periodontitis and to study the effects of A20 overexpression, using a recombinant adenovirus encoding A20 (Ad\u2010A20), on the inflammatory response and on osteoclastic differentiation in lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>)\u2010 and nicotine\u2010stimulated human periodontal ligament cells (<jats:styled-content style=\"fixed-case\">hPDLC<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The concentration of prostaglandin E<jats:sub>2<\/jats:sub> was measured by radioimmunoassay. Reverse transcription\u2013polymerase chain reactions and western blot analyses were used to measure <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein levels, respectively. Osteoclastic differentiation was assessed in mouse bone marrow\u2010derived macrophages using conditioned medium from <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010 and nicotine\u2010treated <jats:styled-content style=\"fixed-case\">hPDLC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A20 was upregulated in the gingival tissues and neutrophils from patients with periodontitis and in <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010 and nicotine\u2010exposed <jats:styled-content style=\"fixed-case\">hPDLC<\/jats:styled-content>s. Pretreatment with A20 overexpression by Ad\u2010A20 markedly attenuated <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010 and nicotine\u2010induced production of prostaglandin E<jats:sub>2<\/jats:sub>, as well as expression of cyclooxygenase\u20102 and proinflammatory cytokines. Moreover, A20 overexpression inhibited the number and size of tartrate\u2010resistant acid phosphatase\u2010stained osteoclasts, and downregulated osteoclast\u2010specific gene expression. <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010 and nicotine\u2010induced p38 phosphorylation and nuclear factor \u03baB activation were blocked by Ad\u2010A20. Ad\u2010A20 inhibited the effects of nicotine and <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> on the activation of pan\u2010protein kinase C, Akt, <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content>\u20103\u03b2 and protein kinase C\u03b1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study is the first to demonstrate that A20 overexpression has anti\u2010inflammatory effects and blocks osteoclastic differentiation in a nicotine\u2010 and <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010stimulated <jats:styled-content style=\"fixed-case\">hPDLC<\/jats:styled-content> model. Thus, A20 overexpression may be a potential therapeutic target in inflammatory bone loss diseases, such as periodontal disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12332","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T12:16:40Z","timestamp":1447071400000},"page":"529-539","source":"Crossref","is-referenced-by-count":31,"title":["Anti\u2010inflammatory and anti\u2010osteoclastogenic effects of zinc finger protein <scp>A<\/scp>20 overexpression in human periodontal ligament cells"],"prefix":"10.1111","volume":"51","author":[{"given":"J.\u2010Y.","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Periodontology School of Dentistry Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.\u2010J.","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology &amp; Research Center for Tooth and Periodontal Tissue Regeneration (MRC) School of Dentistry Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010K.","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry School of Dentistry Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.\u2010T.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Radiology School of Dentistry Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.\u2010C.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology &amp; Research Center for Tooth and Periodontal Tissue Regeneration (MRC) School of Dentistry Kyung Hee University Seoul 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