{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T20:04:49Z","timestamp":1774382689907,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["1933\/12"],"award-info":[{"award-number":["1933\/12"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE 09761"],"award-info":[{"award-number":["DE 09761"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE 022597"],"award-info":[{"award-number":["DE 022597"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["FP7\u2010PEOPLE\u20102011\u2010ITN\u2010290246"],"award-info":[{"award-number":["FP7\u2010PEOPLE\u20102011\u2010ITN\u2010290246"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["FP7\u2010HEALTH\u2010F3\u20102012\u2010306029"],"award-info":[{"award-number":["FP7\u2010HEALTH\u2010F3\u20102012\u2010306029"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Following <jats:italic>Porphyromonas gingivalis<\/jats:italic> infection in mice, the efficacy of vaccination by recombinant and native RgpA in modulating the early local anti\u2010inflammatory and immune responses and periodontal bone loss were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Using the subcutaneous chamber model, exudates were analyzed for cytokines after treatment with native RgpA and adjuvant (test), or adjuvant and saline alone (controls). Mice were also immunized with recombinant RgpA after being orally infected with <jats:italic>P. gingivalis<\/jats:italic>. After 6 wk, serum was examined for anti\u2010<jats:italic>P. gingivalis<\/jats:italic> IgG1 and IgG2a titers and for alveolar bone resorption.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunization with native RgpA shifted the immune response toward an anti\u2010inflammatory response as demonstrated by decreased proinflammatory cytokine <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 production and greater anti\u2010inflammatory cytokine <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 in chamber exudates. Systemically, immunization with recombinant RgpA peptide prevented alveolar bone loss by 50%, similar to immunization with heat\u2010killed whole bacteria. Furthermore, recombinant RgpA shifted the humoral response toward high IgG1 and low IgG2a titers, representing an <jats:italic>in vivo<\/jats:italic> anti\u2010inflammatory response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study demonstrates the potential of RgpA to shift the early local immune response toward an anti\u2010inflammatory response while vaccination with recRgpA protected against <jats:italic>P<\/jats:italic>. gingivalis\u2010induced periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12393","type":"journal-article","created":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T11:53:03Z","timestamp":1465559583000},"page":"285-291","source":"Crossref","is-referenced-by-count":17,"title":["Vaccination with recombinant RgpA peptide protects against <i>Porphyromonas gingivalis<\/i>\u2010induced bone loss"],"prefix":"10.1111","volume":"52","author":[{"given":"A.","family":"Wilensky","sequence":"first","affiliation":[{"name":"Department of Periodontology Faculty of Dental Medicine Hebrew University and Hadassah Medical Center Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Biochemistry Biophysics and Biotechnology Jagiellonian University Krakow Poland"},{"name":"Department of Oral Immunology and Infectious Diseases University of Louisville School of Dentistry Louisville KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Houri\u2010Haddad","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Faculty of Dental Medicine Hebrew University and Hadassah Medical Center Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Shapira","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dental Medicine Hebrew University and Hadassah Medical Center Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1996.1.1.37"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2009.00544.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.080233"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1994.tb00072.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01271-07"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000181144"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01377-08"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5695-5705.2002"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12139"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.01364.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2005.01.001"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/CBI20090290"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510373765"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01036-9"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4493-4501.2001"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1997.tb01189.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.34.21648"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00334.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027009648.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2000.2240108.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4055-4063.2000"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.6.3980"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2480-2486.2002"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.06.013"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(94)90055-8"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.4.1455-1464.1992"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42365-9"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm.1997.378.3-4.223"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01464.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.8.1282"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"78","DOI":"10.4049\/jimmunol.162.1.78","article-title":"Mouse IL\u201013 enhances antibody production in vivo and acts directly on B cells in vitro to increase survival and hence antibody production","volume":"162","author":"Lai YH","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.5.1726"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.12.7959-7963.2001"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200523394"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.9.4108-4114.1998"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.03.013"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12028"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021372"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05056"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02480.x"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00272-09"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740020601"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1993.tb00546.x"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1997.tb00626.x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.10.1629"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.5.2704-2712.2000"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1991.tb00477.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00091.x"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12393","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12393","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T21:26:55Z","timestamp":1696282015000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,10]]},"references-count":49,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1111\/jre.12393"],"URL":"https:\/\/doi.org\/10.1111\/jre.12393","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,10]]}}}