{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:04:55Z","timestamp":1792461895085,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"S\u00e3o Paulo State Research Foundation","award":["2008\/04280-0"],"award-info":[{"award-number":["2008\/04280-0"]}]},{"name":"Amazonas State Research Foundation","award":["014\/2010"],"award-info":[{"award-number":["014\/2010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,4]]},"abstract":"\n Background and Objective<\/jats:title>\n \n No previous study has directly compared the levels of matrix metalloproteinases (\n MMP<\/jats:styled-content>\n s) and tissue inhibitor of metalloproteinases (\n TIMP<\/jats:styled-content>\n s) between smokers and individuals with diabetes mellitus (\n DM<\/jats:styled-content>\n ) with periodontitis. Therefore, the aim of this study was to evaluate the gene expression of\n MMP<\/jats:styled-content>\n \u20101,\n MMP<\/jats:styled-content>\n \u20102,\n MMP<\/jats:styled-content>\n \u20108,\n MMP<\/jats:styled-content>\n \u20109,\n TIMP<\/jats:styled-content>\n \u20101 and\n TIMP<\/jats:styled-content>\n \u20102 in tissues with chronic periodontitis (ChP) of smokers and individuals with type 2\n DM<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Material and Methods<\/jats:title>\n \n Gingival biopsies were harvested from: non\u2010smokers and non\u2010diabetic individuals with ChP (\n n<\/jats:italic>\n = 18) (ChP group); non\u2010diabetic smokers (\u2265 10 cigarettes per day for at least the past 5 years) with ChP (\n n<\/jats:italic>\n = 18) (\n SC<\/jats:styled-content>\n hP group); non\u2010smoking individuals with type 2 diabetes (glycated hemoglobin levels \u2265 7.5%) and ChP (\n n<\/jats:italic>\n = 18) (\n DMC<\/jats:styled-content>\n hP group). The tissue levels of\n mRNA<\/jats:styled-content>\n of\n MMP<\/jats:styled-content>\n \u20101,\n MMP<\/jats:styled-content>\n \u20102,\n MMP<\/jats:styled-content>\n \u20108,\n MMP<\/jats:styled-content>\n \u20109,\n TIMP<\/jats:styled-content>\n \u20101 and\n TIMP<\/jats:styled-content>\n \u20102 were evaluated by quantitative real\u2010time polymerase chain reaction.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n MMP<\/jats:styled-content>\n \u20108 expression was the lowest in the ChP group (\n p<\/jats:italic>\n < 0.05). The\n DMC<\/jats:styled-content>\n hP group presented increased\n mRNA<\/jats:styled-content>\n levels of\n MMP<\/jats:styled-content>\n \u20102 and\n MMP<\/jats:styled-content>\n \u20109, when compared to the\n SC<\/jats:styled-content>\n hP group (\n p<\/jats:italic>\n < 0.05).\n MMP<\/jats:styled-content>\n \u20101 expression and the\n MMP<\/jats:styled-content>\n \u20101\/\n TIMP<\/jats:styled-content>\n \u20101,\n MMP<\/jats:styled-content>\n \u20102\/\n TIMP<\/jats:styled-content>\n \u20101,\n MMP<\/jats:styled-content>\n \u20108\/\n TIMP<\/jats:styled-content>\n \u20101,\n MMP<\/jats:styled-content>\n \u20109\/\n TIMP<\/jats:styled-content>\n \u20101,\n MMP<\/jats:styled-content>\n \u20101\/\n TIMP<\/jats:styled-content>\n \u20102 and\n MMP<\/jats:styled-content>\n \u20102\/\n TIMP<\/jats:styled-content>\n \u20102 ratios were higher in the\n DMC<\/jats:styled-content>\n hP group than in the ChP and\n SC<\/jats:styled-content>\n hP groups (\n p<\/jats:italic>\n < 0.05). The\n DMC<\/jats:styled-content>\n hP group presented lower\n mRNA<\/jats:styled-content>\n levels of\n TIMP<\/jats:styled-content>\n \u20101 than the ChP group (\n p<\/jats:italic>\n < 0.05). The\n MMP<\/jats:styled-content>\n \u20108\/\n TIMP<\/jats:styled-content>\n \u20102 ratio was the highest in the\n SC<\/jats:styled-content>\n hP group (\n p<\/jats:italic>\n < 0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Uncontrolled type 2\n DM<\/jats:styled-content>\n upregulates the ratio of\n MMP<\/jats:styled-content>\n \/\n TIMP<\/jats:styled-content>\n s in sites with ChP more than smoking, which may contribute to a greater extracellular matrix degradation and periodontal breakdown in\n DM<\/jats:styled-content>\n \u2010related periodontitis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jre.12394","type":"journal-article","created":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T07:51:08Z","timestamp":1467359468000},"page":"292-299","source":"Crossref","is-referenced-by-count":33,"title":["Diabetes may affect the expression of matrix metalloproteinases and their inhibitors more than smoking in chronic periodontitis"],"prefix":"10.1111","volume":"52","author":[{"given":"M. F.","family":"Bastos","sequence":"first","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Tucci","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"de Siqueira","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"de Faveri","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. C.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. C.","family":"Vallim","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. M.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00169.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2012.00457.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12063"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12077"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/928315"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.03.007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.07.034"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00663.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01512.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12223"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12194"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12159"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12179"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2010.01659.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090510"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2010.40.1.33"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2010.01705.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2011.41.3.109"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01788.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01375.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2013.43.2.87"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2280(1997)30:2<131::AID-EM5>3.0.CO;2-Q"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01465.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01101.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1014471213371"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2007.06.002"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000070112.80711.3D"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100754"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050293"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01082.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2006.05.014"},{"key":"e_1_2_7_34_1","first-page":"ZC46","article-title":"Effect of smoking on periodontal health","volume":"8","author":"Visvanathan R","year":"2014","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00934.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/000331970505600208"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12177-012-9091-0"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12394","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12394","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T17:26:58Z","timestamp":1696267618000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1111\/jre.12394"],"URL":"https:\/\/doi.org\/10.1111\/jre.12394","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7]]}}}