{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T19:50:07Z","timestamp":1788119407104,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006416","name":"Universidade Estadual de Campinas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Doxycycline is an antibiotic agent that inhibits the activity of matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s) present in the extracellular matrix. In this study, the rat incisor was submitted to a hypofunctional condition, and the effects of doxycycline (80 mg\/kg\/d) on the expression and activity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102, as well as on eruption rate, were determined in the odontogenic region and in the periodontal ligament for 14 d.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Rats were distributed into four groups: normofunctional (<jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>); doxycyline normofunctional (<jats:styled-content style=\"fixed-case\">DNF<\/jats:styled-content>); hypofunctional (<jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content>); and doxycyline hypofunctional (<jats:styled-content style=\"fixed-case\">DHP<\/jats:styled-content>). The left lower incisors of 10 rats were shortened every 2 d, using a high\u2010rotation drill, to produce the <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DHP<\/jats:styled-content> groups, after starting doxycycline treatment (80 mg\/kg) by gavage. Eruption was measured using a millimeter ocular, from the gingival margin to the top of the tooth in the <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DHP<\/jats:styled-content> groups, and also by a mark made in the tooth previously, in the <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DNF<\/jats:styled-content> groups. The hemimandibles were removed and the teeth were extracted to collect the periodontal and odontogenic tissues for immunohistochemical analyses and zymography.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The eruption rates were higher in the <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> and the <jats:styled-content style=\"fixed-case\">DHP<\/jats:styled-content> groups than in the <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DNF<\/jats:styled-content> groups, respectively (<jats:italic>p<\/jats:italic> &lt; 0.05). In the odontogenic region, neither of the treatments changed the expression and activity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102. In the <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> group, the shortening treatment decreased the expression, but not the activity, of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102, while doxycycline was able to inhibit the increase of expression and activity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that the inhibition of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 by doxycycline, during incisor shortening, was not enough to alter the eruption rate, which suggests that <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 may have an important role in the turnover of extracellular matrix of the periodontal ligament during the tooth\u2010eruption process.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12398","type":"journal-article","created":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T11:08:04Z","timestamp":1468580884000},"page":"353-359","source":"Crossref","is-referenced-by-count":12,"title":["Doxycycline reduces the expression and activity of matrix metalloproteinase\u20102 in the periodontal ligament of the rat incisor without altering the eruption process"],"prefix":"10.1111","volume":"52","author":[{"given":"J. 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D.","family":"Novaes","sequence":"additional","affiliation":[{"name":"Universidade Estadual de Campinas Faculdade de Odontologia de Piracicaba Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2007.07.001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.12080"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-010-9295-1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-011-9318-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.3.178"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374980120010501"},{"key":"e_1_2_6_8_1","first-page":"508","article-title":"Doxycycline nano\u2010liposome slow\u2010release gel improves rat periodontitis","volume":"19","author":"Jin HL","year":"2010","journal-title":"Shanghai Kou Qiang Yi Xue."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:HREV.0000011395.11179.af"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.050451"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050344"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110459"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-011-9356-0"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9416(76)90194-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1976.47.7.380"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091890308"},{"key":"e_1_2_6_18_1","first-page":"68","article-title":"An assessment of the control of matrix turnover by a quantitative ultrastructural analysis of fibroblasts of the periodontal ligament in rats","volume":"19","author":"Shore RC1","year":"1991","journal-title":"J Biol Buccale"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb01052.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(74)90249-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22712"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374980120010301"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00893.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2009.07.004"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(84)90088-8"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(01)00102-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1976.tb00057.x"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12398","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12398","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12398","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T13:19:37Z","timestamp":1696252777000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,15]]},"references-count":26,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/jre.12398"],"URL":"https:\/\/doi.org\/10.1111\/jre.12398","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,15]]}}}