{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T12:00:18Z","timestamp":1782993618185,"version":"3.54.5"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,8,6]],"date-time":"2016-08-06T00:00:00Z","timestamp":1470441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC 81271135"],"award-info":[{"award-number":["NSFC 81271135"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Chronic periodontitis causes damage to tooth\u2010supporting tissues, resulting in tooth loss in adults. Recently, cell\u2010sheet\u2010based approaches have been studied to overcome the limitations of conventional cytotherapeutic procedures for periodontal regeneration. The purpose of the present study was to investigate the regenerative potential of periodontal ligament stem cells (<jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s) and human umbilical vein endothelial cells (<jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s) in three\u2010dimensional (3D) cell sheet constructs for periodontal regeneration <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s or co\u2010cultures of both cells were seeded onto temperature\u2010responsive culture dishes, and intact cell sheets were fabricated. Cell sheets were wrapped around the prepared human roots in three different combinations and implanted subcutaneously into immunodeficient mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histological evaluation revealed that after 2, 4 and 8 wk of implantation, periodontal ligament\u2010like tissue arrangements were observed around the implanted roots in experimental groups compared with controls. Vascular lumens were also observed in periodontal compartments of <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>\u2010containing groups. Periodontal ligament regeneration, cementogenesis and osteogenesis were evident in the experimental groups at both weeks 4 and 8, as shown by immunostaining for periostin and bone sialoprotein. Human cells in the transplanted cell sheets were stained by immunohistochemistry for the presence of human mitochondria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The 3D cell sheet\u2010based approach may be potentially beneficial and is thus encouraged for future regenerative periodontal therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12405","type":"journal-article","created":{"date-parts":[[2016,8,6]],"date-time":"2016-08-06T12:13:33Z","timestamp":1470485613000},"page":"408-418","source":"Crossref","is-referenced-by-count":48,"title":["<i>In vivo<\/i> periodontal tissue regeneration by periodontal ligament stem cells and endothelial cells in three\u2010dimensional cell sheet constructs"],"prefix":"10.1111","volume":"52","author":[{"given":"C. P.","family":"Panduwawala","sequence":"first","affiliation":[{"name":"Comprehensive Dental Care (Endodontics) Faculty of Dentistry The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Zhan","sequence":"additional","affiliation":[{"name":"Periodontology Fujian Medical University School of Stomatology Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. L.","family":"Dissanayaka","sequence":"additional","affiliation":[{"name":"Comprehensive Dental Care (Endodontics) Faculty of Dentistry The University of Hong Kong Hong Kong China"},{"name":"HKU Shenzhen Institute of Research and Innovation Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. P.","family":"Samaranayake","sequence":"additional","affiliation":[{"name":"Oral Microbiomics and Infection School of Dentistry University of Queensland Brisbane Qld Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Jin","sequence":"additional","affiliation":[{"name":"Periodontology and Public Health Faculty of Dentistry The University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Comprehensive Dental Care (Endodontics) Faculty of Dentistry The University of Hong Kong Hong Kong China"},{"name":"HKU Shenzhen Institute of Research and Innovation Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00157.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130201300605"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2010.00366.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/14651858"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.00947.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000163546.27639.AA"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1367"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2009.09.003"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2009.00317.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.07.052"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(95)93257-E"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820271005"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(19990615)45:4<355::AID-JBM10>3.0.CO;2-7"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/marc.1990.030111109"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2014.05.024"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.11.036"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000191547.64391.e3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1055"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.12.024"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2183"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.13.16"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12107"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.240309797"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0937635100"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0979"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.05.048"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.31837"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.01.032"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00799.x"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2009.01593.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-010-1009-1"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040455"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00595-011-0106-4"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.088518"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.071"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2009.00312.x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1785"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01597.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2007.01046.x"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.12.017"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.08.008"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2010.0168"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514550040"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1215\/S1152851705000232"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24407"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070624"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10453"},{"key":"e_1_2_8_50_1","first-page":"663","article-title":"Bone sialoprotein gene transfer to periodontal ligament cells may not be sufficient to promote mineralization in vitro or in vivo","volume":"41","author":"Hakki SS","year":"2015","journal-title":"Endod"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199601)244:1<50::AID-AR5>3.0.CO;2-J"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12405","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T13:18:32Z","timestamp":1696252712000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,6]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/jre.12405"],"URL":"https:\/\/doi.org\/10.1111\/jre.12405","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,6]]}}}