{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T11:02:35Z","timestamp":1773054155808,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,24]],"date-time":"2016-09-24T00:00:00Z","timestamp":1474675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>The matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s) play a role in regulating turnover and metabolism of connective tissues in health but they have also been implicated in a wide variety of pathological conditions, including periodontal disease. <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 has been extensively studied in periodontal health and disease using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, although this technique is limited by its inability to determine enzyme activity. The aim was to develop an assay specifically to measure <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 activity and to demonstrate its use in the analysis of gingival crevicular fluid samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>A specific antibody was used to coat black 96\u2010well microtitre plates to capture MMP\u20108 selectively. The activity of bound <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 was measured using a fluorogenic substrate. <jats:styled-content style=\"fixed-case\">Gingival crevicular fluid<\/jats:styled-content> samples, from healthy and periodontally diseased sites, were collected using PerioPaper strips and tested for <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly higher <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 activity was demonstrated in <jats:styled-content style=\"fixed-case\">gingival crevicular fluid<\/jats:styled-content> from periodontally diseased sites compared with healthy sites that exhibited basal or no <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 activity. No cross\u2010reactivity with other <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s was noted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We show, for the first time, that <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 activity can be specifically detected and quantified in <jats:styled-content style=\"fixed-case\">gingival crevicular fluid<\/jats:styled-content> samples. Measurement of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20108 activity could prove to be useful in monitoring periodontal disease progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12423","type":"journal-article","created":{"date-parts":[[2016,9,24]],"date-time":"2016-09-24T07:12:45Z","timestamp":1474701165000},"page":"556-561","source":"Crossref","is-referenced-by-count":23,"title":["<scp>Matrix metalloproteinase<\/scp>\u20108 activity in gingival crevicular fluid: development of a novel assay"],"prefix":"10.1111","volume":"52","author":[{"given":"M. T. C.","family":"Mc Crudden","sequence":"first","affiliation":[{"name":"Centre for Experimental Medicine Queen's University Belfast Belfast UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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