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Elevated <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 levels have been shown in gingival crevicular fluid of patients with chronic periodontitis. The objective of this study was to investigate <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression in chronic periodontitis tissues compared with that in normal tissues. <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression and its inductive mechanism were examined in human gingival epithelial cells (<jats:styled-content style=\"fixed-case\">HGEC<\/jats:styled-content>s) and human gingival fibroblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein were extracted from gingival biopsies of 33 patients with chronic periodontitis and those of 23 healthy volunteers. <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein expression was analyzed by real\u2010time polymerase chain reaction, immunoblotting and immunohistochemistry. <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression in control and stimulated cells in the presence or absence of specific inhibitors for mitogen\u2010activated protein kinase pathways was assayed by real\u2010time polymerase chain reaction, immunoblotting, flow cytometry and immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein expression in chronic periodontitis tissues was significantly greater than that in normal tissues (<jats:italic>p<\/jats:italic> &lt; 0.01). Significantly increased <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression was detected in the gingival epithelium of chronic periodontitis tissues (<jats:italic>p<\/jats:italic> &lt; 0.001). <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">HGEC<\/jats:styled-content>s, but not in human gingival fibroblasts, was significantly induced by stimulation with <jats:italic>Fusobacterium nucleatum<\/jats:italic> (<jats:italic>p<\/jats:italic> &lt; 0.05), partially via the p44\/42 mitogen\u2010activated protein kinase pathway. <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression in the cell lysates and on the surface of <jats:styled-content style=\"fixed-case\">HGEC<\/jats:styled-content>s was induced by stimulation with <jats:italic>F. nucleatum<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression is increased in inflamed chronic periodontitis tissues and localized within gingival epithelium, consistent with an upregulation of <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 expression in <jats:italic>F. nucleatum<\/jats:italic>\u2010stimulated <jats:styled-content style=\"fixed-case\">HGEC<\/jats:styled-content>s, suggesting a possible role of <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>8 in innate immunity of periodontal tissue.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12426","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T09:36:48Z","timestamp":1479375408000},"page":"582-593","source":"Crossref","is-referenced-by-count":8,"title":["Inducible expression of A Disintegrin and Metalloproteinase 8 in chronic periodontitis and gingival epithelial cells"],"prefix":"10.1111","volume":"52","author":[{"given":"W. 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