{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:28:08Z","timestamp":1759177688044},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,17]],"date-time":"2017-03-17T00:00:00Z","timestamp":1489708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Periodontal health is mediated by suppressing microorganisms inducing a local inflammatory host response. Smoking may impair this process. This study compares gingival crevicular fluid levels of inflammatory and bone remodeling markers in heavy smokers and non\u2010smokers following active and supportive periodontal therapy in patients with chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Gingival crevicular fluid and subgingival plaque were collected from the deepest periodontal pocket in 50 patients, 25 smokers and 25 non\u2010smokers, at baseline (T0), following active (T1) and 12 mo of supportive periodontal therapy (T2). Smoking status was validated measuring serum cotinine levels. <jats:styled-content style=\"fixed-case\">Gingival crevicular fluid<\/jats:styled-content> levels of 27 inflammatory and two bone remodeling markers were analyzed using multiplex and singleplex micro\u2010bed immunoassays, and subgingival plaque samples using checkerboard <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> hybridization. Amounts of markers in smokers and non\u2010smokers were compared calculating the effect size.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of inflammatory and bone\u2010remodeling markers in smokers demonstrated an overall reduced effect size at T0 and T2 (<jats:italic>p<\/jats:italic> &lt; 0.001). In particular, proinflammatory markers (<jats:italic>p<\/jats:italic> &lt; 0.001), chemokines (<jats:italic>p<\/jats:italic> = 0.007) and growth factors (<jats:italic>p<\/jats:italic> = 0.003) at T0, osteoprotegerin (<jats:italic>p<\/jats:italic> = 0.003) at T1, proinflammatory markers (<jats:italic>p<\/jats:italic> = 0.019) and chemokines (<jats:italic>p<\/jats:italic> = 0.005) at T2. At T2, <jats:styled-content style=\"fixed-case\">interleukin<\/jats:styled-content>\u20108 was detected in significantly higher levels in smokers. Ten different markers in non\u2010smokers and none in smokers responded to periodontal therapy (<jats:italic>p<\/jats:italic> &lt; 0.05). An overall negative association was revealed between smoking and subgroups of markers at sites presenting \u2265 10<jats:sup>5<\/jats:sup> red complex periodontal microbial species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Except for an upregulation of <jats:styled-content style=\"fixed-case\">interleukin<\/jats:styled-content>\u20108, smokers exhibited reduced <jats:styled-content style=\"fixed-case\">gingival crevicular fluid<\/jats:styled-content> levels of several inflammatory markers at baseline and following active and supportive periodontal therapy. Only inflammatory responses in non\u2010smokers adapted to periodontal therapy. Apparently, there seems to be an immunosuppressant effect of smoking regulating the local inflammatory response and bone remodeling markers captured in <jats:styled-content style=\"fixed-case\">gingival crevicular fluid<\/jats:styled-content> following periodontal therapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12438","type":"journal-article","created":{"date-parts":[[2017,3,17]],"date-time":"2017-03-17T13:46:52Z","timestamp":1489758412000},"page":"713-724","source":"Crossref","is-referenced-by-count":23,"title":["The effect of smoking on inflammatory and bone remodeling markers in gingival crevicular fluid and subgingival microbiota following periodontal therapy"],"prefix":"10.1111","volume":"52","author":[{"given":"D. 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