{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T17:07:49Z","timestamp":1774804069544,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T00:00:00Z","timestamp":1488412800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271149"],"award-info":[{"award-number":["81271149"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Vitamin D\u2010binding protein (<jats:styled-content style=\"fixed-case\">DBP<\/jats:styled-content>) is a highly expressed plasma protein with many important functions, including transport of vitamin <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> metabolites, sequestration of actin, control of bone metabolism and modulation of immune and inflammatory responses. Previous results of our study indicated an association between <jats:styled-content style=\"fixed-case\">DBP<\/jats:styled-content> and periodontitis. We hypothesized that periodontium might be another source of <jats:styled-content style=\"fixed-case\">DBP<\/jats:styled-content> in gingival crevicular fluid other than serum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>DBP expression was examined in dental and periodontal tissues of monkeys by immunohistochemistry, and in primary cells isolated from human dental and periodontal tissues by reverse transcription plus the polymerase chain reaction and immunocytochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DBP was constitutively expressed and widely distributed in dental and periodontal tissues of primates. Their immunoreaction was evident in gingival epithelium, particularly in junctional epithelium, and in mineralizing areas of the dental pulp, periodontal ligament and bone marrow. Correspondingly, m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein expression were detected in primary human gingival epithelial cells, dental pulp cells and periodontal ligament cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DBP is highly expressed and widely distributed in dental and periodontal tissues, which may take an active part in local host defense and hard tissue metabolism.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12445","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T02:42:43Z","timestamp":1488508963000},"page":"755-760","source":"Crossref","is-referenced-by-count":7,"title":["Vitamin <scp>D<\/scp>\u2010binding protein expression in healthy tooth and periodontium: an experimental study both in monkeys <i>in vivo<\/i> and in humans <i>in 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