{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T19:50:07Z","timestamp":1788119407400,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T00:00:00Z","timestamp":1508198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>This study investigated the effect of occlusal contact loss (induced by tooth shortening), on matrix metalloproteinase (<jats:styled-content style=\"fixed-case\">MMP)<\/jats:styled-content>\u20102, membrane type 1\u2010MMP (<jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>1\u2010<jats:styled-content style=\"fixed-case\">MMP)<\/jats:styled-content> and tissue inhibitor of the MMP\u20102 (<jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\u20102) expressions in the periodontal ligament of the rat incisor, as well as eruption rate, resistance and collagen organization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Male Wistar rats were distributed into a control group, denominated normofunctional group, whose lower teeth underwent a normal eruption process; and a hypofunctional group, whose lower left incisor teeth were shortened every 2\u00a0days during 14\u00a0days. Parameters were evaluated on the first, seventh and 14th days and the eruption rate was determined according to the size of the incisor during the eruption process. <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 activity was determined by zymography and the expressions of the <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>1\u2010<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\u20102 proteins were quantitated by western blot. Collagen protein organization, as indicated by the birefringence of the periodontal ligament, was analyzed under polarized light and the periodontal ligament's resistance was determined from the load necessary to inject the incisor into its alveolar space, before extraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Loss of occlusal contact, in rats submitted to hypofunctional eruption, increased <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 activity and eruption rate, but decreased <jats:styled-content style=\"fixed-case\">MT<\/jats:styled-content>1\u2010<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\u20102 expression and disrupted collagen organization in the periodontal ligament, consequently reducing periodontal ligament resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that, after incisor eruption, occlusal contact may be an important factor for regulating the remodeling and the physiological resistance of the periodontal ligament against the continuous eruption process observed in rat incisors.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12484","type":"journal-article","created":{"date-parts":[[2017,10,17]],"date-time":"2017-10-17T06:18:38Z","timestamp":1508221118000},"page":"40-46","source":"Crossref","is-referenced-by-count":7,"title":["Effects of loss of occlusal contact on the expression of matrix metalloproteinase\u20102, membrane type 1\u2010MMP, tissue inhibitor of the MMP\u20102, eruption rate, organization and resistance of collagen fibers of the rat incisor periodontal ligament"],"prefix":"10.1111","volume":"53","author":[{"given":"N. F.","family":"Omar","sequence":"first","affiliation":[{"name":"Departamento de Biologia Estrutural, Molecular e Gen\u00e9tica UEPG  Ponta Grossa PR Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0987-1331","authenticated-orcid":false,"given":"J. R.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Estrutural, Molecular e Gen\u00e9tica UEPG  Ponta Grossa PR Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. S.","family":"Neves","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia \u2013 Histologia FOP\u2010UNICAMP  Piracicaba SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. D.","family":"Novaes","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia \u2013 Histologia FOP\u2010UNICAMP  Piracicaba SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(81)90084-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.04.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-011-9356-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-010-9295-1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-011-9318-6"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22712"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/341905"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760100701"},{"key":"e_1_2_7_10_1","first-page":"206","article-title":"How teeth erupt","volume":"17","author":"Berkovitz BK","year":"1990","journal-title":"Dent Update"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345870660050201"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9416(76)90194-9"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20067"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00348.2011"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22085"},{"key":"e_1_2_7_16_1","first-page":"643","article-title":"Effects of continuous and interrupted forces on gene transcription in periodontal ligament cells in vitro","volume":"49","author":"Hacopian N","year":"2011","journal-title":"Acta Med Iran"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1998.tb02350.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00782.x"},{"key":"e_1_2_7_19_1","first-page":"116","article-title":"Effects of mechanical stress on the expression of type XII collagen mRNA in human periodontal ligament cells","volume":"10","author":"Uno K","year":"2001","journal-title":"J Hard Tissue Biol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2010.08.004"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-11-10"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2011.0367"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2011.01423.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1600243"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.4.2446"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0350704"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.3.615"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02723-5"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.31.21491"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000070112.80711.3D"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.17.4782"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.12080"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0722.2002.21384.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008200390181816"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-006-9060-7"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1998.tb02350.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12398"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(99)00136-3"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2006.00326.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2011.00856.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(82)90049-8"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(82)90045-0"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/21.1.49"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(88)90102-1"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(03)00010-4"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207509152184"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(82)90044-9"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092270402"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2009.42.7.427"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12028"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2009.01597.x"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.2.871"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12484","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12484","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jre.12484","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12484","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T16:02:07Z","timestamp":1693670527000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12484"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,17]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/jre.12484"],"URL":"https:\/\/doi.org\/10.1111\/jre.12484","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,17]]}}}