{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T19:34:25Z","timestamp":1778873665159,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:00:00Z","timestamp":1508803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grants-in-Aid for Scientific Research","award":["JP15K11385"],"award-info":[{"award-number":["JP15K11385"]}]},{"name":"Grants-in-Aid for Scientific Research","award":["JP15H02579"],"award-info":[{"award-number":["JP15H02579"]}]},{"name":"Grants-in-Aid for Scientific Research","award":["JP17H06853"],"award-info":[{"award-number":["JP17H06853"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Single nucleotide polymorphisms (<jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s) of paraoxonase 1 (<jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1) are known to be associated with the pathogenesis of osteoporosis and periodontitis. However, the effects of <jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1 on the osteoblastic differentiation of periodontal ligament (<jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content>) cells are unclear. In this study, we examined the effects of <jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1 on the osteoblastic differentiation of <jats:styled-content style=\"fixed-case\">PDL<\/jats:styled-content> cells, and analysed the role of <jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1 <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>s on the pathogenesis of aggressive periodontitis (AgP) in the Japanese population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Human PDL (HPDL) cells were exposed to the PON1 plasmid and PON1 inhibitor, 2\u2010hydroxyquinoline, and cultured in mineralization medium. Expression of calcification\u2010related genes and calcified nodule formation were assessed by real\u2010time PCR, an alkaline phosphatase (ALPase) activity assay and Alizarin red staining. Sanger sequencing was performed to evaluate whether PON1 SNPs are associated with the pathogenesis of AgP in Japanese people.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>During osteoblastic differentiation of HPDL cells, expression of PON1 mRNA increased in a time\u2010dependent manner. PON1 stimulated an increase in expression of mRNA for calcification\u2010related genes, as well as ALPase activity. In contrast, 2\u2010hydroxyquinoline clearly inhibited the expression of calcification\u2010related genes, ALPase activity and calcified nodule formation in HPDL cells. Moreover, there was a statistically significant difference in the minor allele frequency of PON1 SNP rs854560 between the Japanese control database and patients with AgP in the Japanese population (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.0190).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1 induced cytodifferentiation and mineralization of <jats:styled-content style=\"fixed-case\">HPDL<\/jats:styled-content> cells, and <jats:styled-content style=\"fixed-case\">PON<\/jats:styled-content>1 <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> rs854560 may be associated with the pathogenesis of AgP in the Japanese population.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12507","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T07:02:46Z","timestamp":1508828566000},"page":"200-209","source":"Crossref","is-referenced-by-count":5,"title":["Effects of paraoxonase 1 on the cytodifferentiation and mineralization of periodontal ligament cells"],"prefix":"10.1111","volume":"53","author":[{"given":"R.","family":"Masumoto","sequence":"first","affiliation":[{"name":"Division of Oral Biology and Disease Control Department of Periodontology Osaka University Graduate School of Dentistry  Suita Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3613-6677","authenticated-orcid":false,"given":"J.","family":"Kitagaki","sequence":"additional","affiliation":[{"name":"Division of Oral Biology and Disease Control Department of Periodontology Osaka University Graduate School of Dentistry  Suita 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