{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:40:25Z","timestamp":1785559225028,"version":"3.56.0"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,28]],"date-time":"2017-10-28T00:00:00Z","timestamp":1509148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670995"],"award-info":[{"award-number":["81670995"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371146"],"award-info":[{"award-number":["81371146"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81503624"],"award-info":[{"award-number":["81503624"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Interleukin\u201022 (IL\u201022), mainly produced by CD4+ T\u2010helper subtypes and innate lymphoid cells at barrier surfaces, is found to involve in several diseases, including diabetes, rheumatoid arthritis, peri\u2010implantitis and chronic rhinosinusitis with nasal polyps. The purpose of this study was to investigate histological changes and the levels of interleukin\u201022, interleukin\u201022 receptor 1 (IL\u201022R1) and interleukin\u201022\u2010binding protein (IL\u201022BP) in experimental periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Sixty male 8\u2010week\u2010old Sprague Dawley rats were randomly allocated to six groups of 10 rats each. In the periodontitis groups, experimental periodontitis was established and the rats were killed on days 3, 5, 7, 11 and 15 after ligation, while the rats without ligation were killed on day 0, representing the healthy control group (day 0 group). Histopathologic changes were detected by hematoxylin and eosin (H&amp;E) staining, and alveolar bone loss was determined by micro\u2010computed tomography (micro\u2010CT). Tartrate\u2010resistant acid phosphatase (TRAP) staining was used to investigate osteoclast formation. Real\u2010time quantitative PCR (qPCR) and immunohistochemistry were used to investigate the expression and location of IL\u201022, IL\u201022R1 and IL\u201022BP in gingival tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>H&amp;E staining showed increasingly severe destruction of the epithelial layer between day 3 and day 7, and the hyperplasia of pocket epithelium and the formation of periodontal pockets could be detected from day 11 to day 15. Micro\u2010CT indicated an exponential increase in alveolar bone loss from day 3 to day 11 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). Bone resorption tended to be stationary after this period. TRAP staining showed that the number of multinucleate osteoclasts peaked at day 3 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001, compared with day 0) and decreased at subsequent time points between day 5 and day 15. IL\u201022BP was expressed strongly under steady\u2010state conditions in epithelial cells. IL\u201022\u2010positive cells could be clearly observed both in the epithelial layer and around the lamina propria, whereas IL\u201022R1 was mainly localized in the epithelial layer of the damage period. Real\u2010time qPCR revealed up\u2010regulation of IL\u201022 and IL\u201022R1, as well as down\u2010regulation of IL\u201022BP in gingival tissues during the destructive phase of periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study shows the expression and localization of IL\u201022, IL\u201022R1 and IL\u201022BP, as well as the relevant histopathological alterations during the development of experimental periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12512","type":"journal-article","created":{"date-parts":[[2017,10,28]],"date-time":"2017-10-28T05:02:35Z","timestamp":1509166955000},"page":"250-257","source":"Crossref","is-referenced-by-count":12,"title":["Temporal expression of interleukin\u201022, interleukin\u201022 receptor 1 and interleukin\u201022\u2010binding protein during experimental periodontitis in rats"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5809-333X","authenticated-orcid":false,"given":"S.","family":"Pan","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Yang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Zhang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Zhang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Zhang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Liu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Li","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University  Wuhan China"},{"name":"Department of Periodontology, School and Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.10.1528"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb02310.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/idj.12185"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01311.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13564"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000002137"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.08.006"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e31811edd4f"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.06.008"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101312"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1720"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007837200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.08.002"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6363947"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12027"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12019"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2007.00989.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.03.024"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032414-112123"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12643"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/801974"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154904"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11535"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01269.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.11.003"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.6.3635"},{"key":"e_1_2_6_28_1","first-page":"45","article-title":"Early steps in bone resorption in experimental periodontitis: a histomorphometric study","volume":"7","author":"Ubios AM","year":"1993","journal-title":"Acta Odontol Latinoam"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T04:06:22Z","timestamp":1695614782000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,28]]},"references-count":27,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/jre.12512"],"URL":"https:\/\/doi.org\/10.1111\/jre.12512","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,28]]}}}