{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T07:52:55Z","timestamp":1740124375134,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T00:00:00Z","timestamp":1514332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"New Zealand Dental Association Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>The salivary transcriptome may present as a readily available and non\u2010invasive source of potential biomarkers. The development of chronic periodontitis is determined by individual patient susceptibility; hence, the aim of this study was to determine the potential of the salivary transcriptome as a biomarker of disease susceptibility using chronic periodontitis as an example.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Using an Oragene<jats:sup>\u00ae<\/jats:sup> <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> kit, the total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was purified from the saliva of 10 patients with chronic periodontitis and 10 patients without chronic periodontitis. The quantity and quality of the total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was determined, and a measure of gene expression via <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> was undertaken using the Affymetrix microarray system. The microarray profiling result was further validated by real\u2010time quantitative <jats:styled-content style=\"fixed-case\">polymerase chain reaction<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Spectrophotometric analysis showed the total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> purified from each participant ranged from 0.92\u00a0\u03bcg\/500\u00a0\u03bcL to 62.85\u00a0\u03bcg\/500\u00a0\u03bcL. There was great variability in the quantity of total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> obtained from the 2 groups in the study with a mean of 10.21\u00a0\u00b1\u00a012.71\u00a0\u03bcg\/500\u00a0\u03bcL for the periodontitis group and 15.97\u00a0\u00b1\u00a023.47\u00a0\u03bcg\/500\u00a0\u03bcL for the control group. Further the <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> purity (based on the A<jats:sub>260<\/jats:sub>\/A<jats:sub>280<\/jats:sub> ratio) for the majority of participants (9 periodontitis and 6 controls) were within the acceptable limits for downstream analysis (2.0\u00a0\u00b1\u00a00.1). The study samples, showed 2 distinct bands at 23S (3800\u00a0bp) and 16S (1500\u00a0bp) characteristic of bacterial <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content>. Preliminary microarray analysis was performed for 4 samples (P2, P6, H5 and H9). The percentage of genes present in each of the 4 samples was not consistent with about 1.8%\u201018.7% of genes being detected. Quantitative real\u2010time polymerase chain reaction confirmed that the total <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> purified from each sample was mainly bacterial <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (Uni 16S) with minimal human <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study showed that minimal amounts of human <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> were able to be isolated from the saliva of patients with periodontitis as well as controls. Further work is required to enhance the extraction process of human <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> from saliva if the salivary transcriptome is to be used in determining individual patient susceptibility.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12522","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T06:26:40Z","timestamp":1514356000000},"page":"369-377","source":"Crossref","is-referenced-by-count":5,"title":["Feasibility of the salivary transcriptome as a novel biomarker in determining disease susceptibility"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0754-6204","authenticated-orcid":false,"given":"M. F. 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