{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T12:40:19Z","timestamp":1789130419622,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004048","name":"Kashan University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004048","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010146a (miR\u2010146a) is a small noncoding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> that plays a critical role in the negative regulation of the innate immune response, and the dysregulation of miR\u2010146a has been associated with several inflammatory disorders. In generalized aggressive periodontitis (GAgP) the degree of clinical inflammation appears to be similar to that of chronic periodontitis, and, in this situation, age of onset and family history are important additional criteria for diagnosis. This study was performed to evaluate the level of miR\u2010146a expressed in gingival tissues of patients with <jats:styled-content style=\"fixed-case\">GA<\/jats:styled-content>gP and its association with disease severity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Gingival samples from 18 patients with <jats:styled-content style=\"fixed-case\">GA<\/jats:styled-content>gP and 10 healthy subjects were collected and the level of miR\u2010146a and its targets, including necrosis factor\u2010alpha, interleukin\u20101beta, and interleukin\u20106, were assessed using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><jats:p>Clinical parameters, including probing depth and clinical attachment loss, were measured and their correlations with the level of miR\u2010146a were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results demonstrated an elevation in the level of miR\u2010146a expressed in patients with <jats:styled-content style=\"fixed-case\">GA<\/jats:styled-content>gP compared with healthy controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001), which was directly associated with disease severity (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Overexpression of miR\u2010146a was accompanied by a reduction in the levels of pro\u2010inflammatory cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that there is an association between miR\u2010146a and <jats:styled-content style=\"fixed-case\">GA<\/jats:styled-content>gP and imply that miR\u2010146a may serve as an indicator of periodontal disease severity. However, further studies and additional information are required to confirm this relationship and the precise role of miR\u2010146a in the development and\/or progression of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12538","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T09:59:32Z","timestamp":1539770372000},"page":"27-32","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Assessment of micro<scp>RNA<\/scp>\u2010146a in generalized aggressive periodontitis and its association with disease severity"],"prefix":"10.1111","volume":"54","author":[{"given":"S.","family":"Ghotloo","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Kashan University of Medical Sciences  Kashan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Motedayyen","sequence":"additional","affiliation":[{"name":"Department of Laboratory 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