{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T03:05:00Z","timestamp":1786763100381,"version":"build-2736575974"},"reference-count":71,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,4,23]],"date-time":"2018-04-23T00:00:00Z","timestamp":1524441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Background and Objectives<\/jats:title>Determine the presence of mesenchymal stem cells (MSC<\/jats:styled-content>s) in healthy periodontal tissue and periodontal granulation tissue (GT<\/jats:styled-content>) and explore associations between immuno\u2010regulatory molecules and selected subgingival microorganisms.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>Mesenchymal stem cells were isolated, propagated and characterised by flow cytometry from a region of healthy gingival tissue and inflamed GT<\/jats:styled-content> of 10 systemically healthy non\u2010smokers with chronic periodontitis. Tissue levels of immunoregulatory molecules were determined by qPCR<\/jats:styled-content> and Gingival Crevicular Fluid (GCF<\/jats:styled-content>) levels by ELISA<\/jats:styled-content>. Subgingival plaque levels of periodontal pathogens were determined by qPCR<\/jats:styled-content><\/jats:p><\/jats:sec>Results<\/jats:title>Cells with MSC<\/jats:styled-content>\u2010properties were isolated from both inflamed GT<\/jats:styled-content> and healthy gingival (G) tissue. A pro\u2010inflammatory process predominated in GT<\/jats:styled-content> which was partly reflected in GCF<\/jats:styled-content> and putative periodontal pathogens were higher at diseased sites. However, there was no significant difference in surface levels of mesenchymal (CD<\/jats:styled-content>90, CD<\/jats:styled-content>73, CD<\/jats:styled-content>146, CD<\/jats:styled-content>271, STRO<\/jats:styled-content>\u20101), endothelial (CD<\/jats:styled-content>105, CD<\/jats:styled-content>106), hematopoietic (CD<\/jats:styled-content>34, CD<\/jats:styled-content>45) and embryonic (SSEA<\/jats:styled-content>\u20104) stem cell markers between MSC<\/jats:styled-content>s isolated from GT<\/jats:styled-content> and G tissue.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Periodontal lesions, albeit inflamed, retain healing potential as inferred by the presence of MSC<\/jats:styled-content>\u2010like cells with similar immunophenotypic characteristics to those found in healthy periodontal tissue. Therefore, there might be merits for healing in preserving sufficient GT<\/jats:styled-content> in\u2010situ during periodontal surgery.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12551","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T04:09:32Z","timestamp":1524542972000},"page":"610-621","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Stem cell\u2010like populations and immunoregulatory molecules in periodontal granulation tissue"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4972-9532","authenticated-orcid":false,"given":"D. 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