{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:15:53Z","timestamp":1780676153022,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,23]],"date-time":"2018-04-23T00:00:00Z","timestamp":1524441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008968","name":"National Research Council Sri Lanka","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Human beta\u2010defensins (<jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>s) contribute to innate immunity antimicrobial activity. They are also effective in the adaptive immune response and may play a crucial role in the susceptibility to diseases of the oral cavity. This study aimed to evaluate the levels of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 in the gingival crevicular fluid of individuals with and without chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Twenty periodontally healthy individuals (H) and 20 individuals with chronic periodontitis were recruited. <jats:styled-content style=\"fixed-case\">Gingival crevicular fluid<\/jats:styled-content> samples were collected from: healthy sites (Hh) from periodontally healthy individuals; and healthy sites (Ph), sites with gingivitis (Pg), and sites with periodontitis (Pp) from individuals with periodontitis. The levels of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 (pg\/mL) were measured using <jats:styled-content style=\"fixed-case\">enzyme\u2010linked immunosorbent assay<\/jats:styled-content>. Comparisons of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 between individuals (H and chronic periodontitis) and among sites (Hh, Ph, Pg, Pp) were performed through hierarchical linear modeling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">Gingival crevicular fluid<\/jats:styled-content> levels of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 were: Hh\u00a0=\u00a0229.52\u00a0\u00b1\u00a0138.96 (median 199.26), Ph\u00a0=\u00a053.88\u00a0\u00b1\u00a058.17 (median 35.75), Pg\u00a0=\u00a057.11\u00a0\u00b1\u00a040.18 (median 39.90) and Pp\u00a0=\u00a055.31\u00a0\u00b1\u00a037.28 (median 54.19). No influence of site diagnosis (level 1; health\/gingivitis\/periodontitis) was observed; however, individual diagnosis (level 2; health\/periodontitis) influenced the levels of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Periodontally healthy individuals showed higher <jats:styled-content style=\"fixed-case\">gingival crevicular fluid<\/jats:styled-content> levels of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 when compared to individuals with chronic periodontitis. This suggests a potential protective role of <jats:styled-content style=\"fixed-case\">hBD<\/jats:styled-content>\u20101 in the susceptibility to chronic periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12558","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T04:15:37Z","timestamp":1524543337000},"page":"736-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Gingival crevicular fluid levels of human beta\u2010defensin 1 in individuals with and without chronic periodontitis"],"prefix":"10.1111","volume":"53","author":[{"given":"L. C. M.","family":"Costa","sequence":"first","affiliation":[{"name":"Department of Dental Clinics Oral Pathology, and Oral Surgery School of Dentistry Federal University of Minas Gerais\u2010 UFMG  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. R.","family":"Soldati","sequence":"additional","affiliation":[{"name":"Departament of Diagnosis and Surgery School of Dentistry S\u00e3o Paulo State University\u2010UNESP  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. C.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Dentistry University Center of Lavras\u2010UNILAVRAS  Lavras Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Dental Clinics Oral Pathology, and Oral Surgery School of Dentistry Federal University of Minas Gerais\u2010 UFMG  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. H. N. G.","family":"Abreu","sequence":"additional","affiliation":[{"name":"Department of Community and Preventive Dentistry School of Dentistry Federal University of Minas Gerais\u2010UFMG  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7687-1238","authenticated-orcid":false,"given":"F. O.","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Dental Clinics Oral Pathology, and Oral Surgery School of Dentistry Federal University of Minas Gerais\u2010 UFMG  Belo Horizonte Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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