{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T14:37:58Z","timestamp":1778769478170,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,6,14]],"date-time":"2018-06-14T00:00:00Z","timestamp":1528934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371146"],"award-info":[{"award-number":["81371146"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670995"],"award-info":[{"award-number":["81670995"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,10]]},"abstract":"Background and Objectives<\/jats:title>In periodontitis, the Wnt\/\u03b2\u2010catenin signaling pathway is related to the metabolism of the alveolar bone; further, extracellular matrix metalloproteinase inducer (EMMPRIN<\/jats:styled-content>) expression is correlated with matrix metalloproteinases (MMP<\/jats:styled-content>s) expression and inflammation severity. The aim of this study was to perform a preliminary investigation of the interaction between the Wnt\/\u03b2\u2010catenin signaling pathway and the EMMPRIN<\/jats:styled-content>\/MMP<\/jats:styled-content>s route in periodontitis.<\/jats:p><\/jats:sec>Material and Methods<\/jats:title>Chronic periodontitis and healthy gingival tissues were obtained to detect the expression of Wnt3a, \u03b2\u2010catenin, EMMPRIN<\/jats:styled-content> and MMP<\/jats:styled-content>\u20102, 9 by using immunohistochemical analysis. The human immortalized oral epithelial cell\/human gingival fibroblast direct co\u2010culture model was treated with 10\u00a0\u03bcg\/mL<\/jats:styled-content> Porphyromonas gingivalis lipopolysaccharide (Pg. LPS<\/jats:styled-content>). Anti\u2010EMMPRIN<\/jats:styled-content> antibody was used to block the effect of EMMPRIN<\/jats:styled-content>. Dickkopf\u20101 (DKK<\/jats:styled-content>\u20101) and Wnt3a were used as the inhibitor and activator of the Wnt\/\u03b2\u2010catenin signaling pathway, respectively. Immunofluorescence was performed to visualize the localization of \u03b2\u2010catenin and EMMPRIN<\/jats:styled-content>. Expression of the EMMPRIN<\/jats:styled-content>, MMP<\/jats:styled-content>\u20102, 9 and Wnt pathway's components was confirmed by western blotting and quantitative real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec>Results<\/jats:title>Higher levels of Wnt3a, \u03b2\u2010catenin, EMMPRIN<\/jats:styled-content> and MMP<\/jats:styled-content>\u20102, 9 were observed in chronic periodontitis gingival tissues compared with controls. Pg. LPS<\/jats:styled-content> significantly enhanced \u03b2\u2010catenin, p\u2010GSK<\/jats:styled-content>\u20103\u03b2, EMMPRIN<\/jats:styled-content> and MMP<\/jats:styled-content>\u20102, 9 inductions in the human immortalized oral epithelial cell\/human gingival fibroblast co\u2010culture model. Anti\u2010EMMPRIN<\/jats:styled-content> antibody markedly reduced the expression of MMP<\/jats:styled-content>\u20102, 9 only in the presence of Pg. LPS<\/jats:styled-content>. Co\u2010expression of \u03b2\u2010catenin and EMMPRIN<\/jats:styled-content> was detected in the co\u2010culture model. DKK<\/jats:styled-content>\u20101 inhibited Wnt pathway, but upregulated the EMMPRIN<\/jats:styled-content>\/MMP<\/jats:styled-content>\u20102, 9 routes. In contrast, activating Wnt pathway by Wnt3a repressed the EMMPRIN<\/jats:styled-content>\/MMP<\/jats:styled-content>\u20102, 9 routes. The promotion effect of DKK<\/jats:styled-content>\u20101 on MMP<\/jats:styled-content>\u20102, 9 expressions was partially inhibited by the anti\u2010EMMPRIN<\/jats:styled-content> antibody. In addition, anti\u2010EMMPRIN<\/jats:styled-content> antibody led to a drastic decrease in \u03b2\u2010catenin and p\u2010GSK<\/jats:styled-content>\u20103\u03b2.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In periodontitis, EMMPRIN<\/jats:styled-content> regulates MMP<\/jats:styled-content>\u20102, 9 expressions, the activation of Wnt\/\u03b2\u2010catenin signaling pathway downregulates the EMMPRIN<\/jats:styled-content>\/MMP<\/jats:styled-content>\u20102, 9 routes and the blockade of EMMPRIN<\/jats:styled-content> attenuates Wnt\/\u03b2\u2010catenin signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12574","type":"journal-article","created":{"date-parts":[[2018,6,14]],"date-time":"2018-06-14T07:28:50Z","timestamp":1528961330000},"page":"842-852","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Interaction between the Wnt\/\u03b2\u2010catenin signaling pathway and the EMMPRIN<\/scp>\/MMP<\/scp>\u20102, 9 route in periodontitis"],"prefix":"10.1111","volume":"53","author":[{"given":"X.","family":"Liu","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Zhang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Pan","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Shang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan Hubei China"},{"name":"Department of Periodontology School and Hospital of Stomatology Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0494-4258","authenticated-orcid":false,"given":"C.","family":"Li","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of Stomatology Wuhan University Wuhan Hubei China"},{"name":"Department of Periodontology School and Hospital of Stomatology Wuhan University Wuhan Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,6,14]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"203","article-title":"Periodontitis: a host\u2010mediated disruption of microbial homeostasis. 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