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The aim of the present study was to collect in vivo and in silico evidence on the role of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 in the interplay between T2<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content> and generalized periodontitis (GP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The extent of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 expression and localization were immunohistochemically analyzed in gingival tissues of patients with GP with T2<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content> (T2<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>, n\u00a0=\u00a011), systemically healthy patients with <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content> (n\u00a0=\u00a07), and systemically and periodontally healthy controls (n\u00a0=\u00a011). An in silico network model was built to determine the interactions between <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 and T2<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content> pathways. Regulation of neutrophil transmigration by <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 was analyzed in a knock\u2010out mice model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In human gingival tissues, the proportion of cells with robust <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 expression was elevated in patients with T2<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content> in comparison to controls (<jats:italic>P\u00a0<\/jats:italic>=\u00a0.014). According to the in silico analysis, \u201chydroxyl radical\u201d and \u201chydrogen peroxide\u201d compounds were among the most central nodes of the network, and were within the shortest paths connecting \u201cglucose\u201d to \u201c<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7.\u201d In <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 knock\u2010out mice, an intense accumulation of neutrophils was observed in the gingival epithelium as compared to wild\u2010type mice (<jats:italic>P\u00a0<\/jats:italic>=\u00a0.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Elevated <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 expression in gingival tissues of patients with T2<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content> is related to the activation of reactive oxygen species by hyperglycemia. Suppression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>7 expression results in impaired neutrophil transmigration in gingiva.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12583","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T06:20:22Z","timestamp":1530771622000},"page":"916-923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["<scp>Matrix metalloproteinase\u2010<\/scp>7 in periodontitis with type 2 diabetes mellitus"],"prefix":"10.1111","volume":"53","author":[{"given":"F.","family":"Zeid\u00e1n\u2010Chuli\u00e1","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas, Bioqu\u00edmica Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul (UFRGS)  Porto Alegre RS Brazil"},{"name":"Department of Periodontology Institute of Dentistry University of Turku  Turku Finland"},{"name":"Departamento de Ciencias Biom\u00e9dicas B\u00e1sicas Facultad de Ciencias Biom\u00e9dicas y de la Salud Universidad Europea de Madrid  Villaviciosa de Od\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Periodontology Institute of Dentistry University of Turku  Turku Finland"},{"name":"Department of Periodontology Faculty of Dentistry University of Sakarya  Sakarya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"H\u00e4kkinen","sequence":"additional","affiliation":[{"name":"Laboratory of Periodontal Biology Faculty of Dentistry University of British Columbia  Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"K\u00f6n\u00f6nen","sequence":"additional","affiliation":[{"name":"Department of Periodontology Institute of Dentistry University of Turku  Turku Finland"},{"name":"Oral Health Care Welfare Division City of Turku  Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.\u2010H.","family":"Neves de Oliveira","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas, Bioqu\u00edmica Departamento de Bioqu\u00edmica Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade Universidade Federal do Rio Grande do Sul (UFRGS)  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"G\u00fcnc\u00fc","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry University of Hacettepe  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.\u2010J.","family":"Uitto","sequence":"additional","affiliation":[{"name":"Department of Oral Biology Institute of Dentistry University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Caglayan","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry University of Hacettepe  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1225-5751","authenticated-orcid":false,"given":"U. 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