{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:17:04Z","timestamp":1790252224251,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T00:00:00Z","timestamp":1537142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2014HP019"],"award-info":[{"award-number":["ZR2014HP019"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003091","name":"Shandong Province","doi-asserted-by":"publisher","award":["2015WS0041"],"award-info":[{"award-number":["2015WS0041"]}],"id":[{"id":"10.13039\/501100003091","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012620","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["20120233"],"award-info":[{"award-number":["20120233"]}],"id":[{"id":"10.13039\/100012620","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Periodontitis is a chronic inflammatory disease characterized by the destruction of the periodontium. The strontium ion (Sr<jats:sup>2+<\/jats:sup>) can prevent the bone loss associated with periodontitis and promote the regeneration of the bone. The mechanisms by which the Sr<jats:sup>2+<\/jats:sup> works remain poorly understood. We aim to investigate the effects of the Sr<jats:sup>2+<\/jats:sup> ion on cell proliferation, inflammatory regulation and osteogenic differentiation of human periodontal ligament cells (hPDLCs) in pathological conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>hPDLCs were obtained from premolars that came from the orthodontic extraction. The hPDLCs were treated with Sr<jats:sup>2+<\/jats:sup> and\/or lipopolysaccharide (LPS), which was applied as the pathological condition of periodontitis. The effect of the dose of Sr<jats:sup>2+<\/jats:sup> on cell proliferation was analyzed using a Cell Counting Kit\u20108 assay. The gene and protein expression of proinflammatory cytokines were detected by the real\u2010time polymerase chain reaction and enzyme\u2010linked immunosorbent assay. The osteogenic differentiation and mineralization were assessed by the real\u2010time polymerase chain reaction, alkaline phosphatase activity assay and alizarin red staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Results demonstrated that Sr<jats:sup>2+<\/jats:sup> in a range of concentrations from 0.02 to 2.5\u00a0mmol\/L significantly improved the proliferation of hPDLCs. Sr<jats:sup>2+<\/jats:sup> reversed LPS\u2010stimulated proinflammatory cytokine expressions such as tumor necrosis factor alpha, interleukin (IL)\u20101\u03b2, IL\u20106 and IL\u20108. Moreover, Sr<jats:sup>2+<\/jats:sup> rescued the LPS\u2010inhibited gene expression of osteogenic differentiation. Although it appeared to suppress the late mineralization, Sr<jats:sup>2+<\/jats:sup> can reverse the LPS\u2010inhibited early osteogenic differentiation of hPDLCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results indicated that Sr<jats:sup>2+<\/jats:sup> could attenuate the LPS\u2010stimulated proinflammatory molecule expression and inhibit early osteogenic differentiation of hPDLCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12599","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T08:29:10Z","timestamp":1537172950000},"page":"999-1008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Strontium ion attenuates lipopolysaccharide\u2010stimulated proinflammatory cytokine expression and lipopolysaccharide\u2010inhibited early osteogenic differentiation of human periodontal ligament cells"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5035-4817","authenticated-orcid":false,"given":"Lingfei","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Oral Implantology Yantai Stomatological Hospital  Yantai China"},{"name":"Department of Oral Implantology and Prosthetic Dentistry Academic Center for Dentistry Amsterdam (ACTA)  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Periodontology Yantai Stomatological Hospital  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6762-9303","authenticated-orcid":false,"given":"Wenjuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oral Implantology Yantai Stomatological Hospital  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shutai","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Periodontology Yantai Stomatological Hospital  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuelian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Implantology and Prosthetic Dentistry Academic Center for Dentistry Amsterdam (ACTA)  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Rausch\u2010Fan","sequence":"additional","affiliation":[{"name":"Division of conservative Dentistry and Periodontology and Competence Center of Periodontal Research Vienna Dental School Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Implantology Yantai Stomatological Hospital  Yantai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11-S.2033"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2017.170210"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2013.11.008"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425915616685"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514547762"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.32066"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/scrt456"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-015-0824-y"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12193"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejh.2017.2791"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2014.10.001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-6646(08)60178-3"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aanat.2011.09.008"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.160227"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.06.023"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104527"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515618887"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2012.1031"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022436"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2007.04.020"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000327950"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2008.05.001"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25904"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2017.01.007"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21359"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2012.04.045"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36251"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2009.0405"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2012.07.009"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.251116"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/871863"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-014-2791-5"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-013-9698-6"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2079"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12599","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T17:38:45Z","timestamp":1694885925000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,17]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1111\/jre.12599"],"URL":"https:\/\/doi.org\/10.1111\/jre.12599","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,17]]},"assertion":[{"value":"2018-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}