{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:34:10Z","timestamp":1761744850357,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102015R1A2A2A01004589","NRF\u20102016M1A5A1027461","NRF\u20102016M3A9B6026771"],"award-info":[{"award-number":["NRF\u20102015R1A2A2A01004589","NRF\u20102016M1A5A1027461","NRF\u20102016M3A9B6026771"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Proteome analysis of periodontal ligament stem cells (PDLSCs) could be used to study the function of PDL tissue. We used a label\u2010free quantitative proteomic technique to investigate differentially expressed proteins (DEPs) in human PDLSCs (hPDLSCs) compared to human bone marrow mesenchymal stem cells (hBMSCs) and identify proteins specific to hPDLSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>hPDLSCs (n\u00a0=\u00a03) and hBMSCs (n\u00a0=\u00a03) were cultured and harvested for protein extraction and trypsin digestion. The proteomes of both cell types were analyzed by nano\u2010liquid chromatography\/tandem mass spectrometry. DEPs in hPDLSCs compared to hBMSCs were detected by label\u2010free quantification and evaluated through signal transduction pathway and gene ontology (GO) analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 690 and 771 proteins were identified from hPDLSCs and hBMSCs, of which 561 proteins were in common and 124 DEPs were found between hPDLSCs and hBMSCs. Fifty\u2010eight proteins were expressed at significantly higher levels in hPDLSCs, whereas 66 proteins were expressed at lower levels compared to hBMSCs. The more highly expressed proteins were associated with translation and initiating protein synthesis, and lower expressed proteins were related to cell aging and metabolic processes. Proteins unique to hPDLSCs and hBMSCs were associated with translation and metabolic processes, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate evidence of distinct differences in protein expression between hPDLSCs and hBMSCs by using label\u2010free quantitative proteomic analysis which was the first attempt in this field. DEPs included previously reported hPDLSC marker proteins and novel marker candidates, such as microtubule\u2010associated protein, CTP synthase 1 and stathmin, which could be the markers for developing periodontal disease diagnostics and therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12604","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T06:43:41Z","timestamp":1539067421000},"page":"53-62","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Label\u2010free quantitative proteomic analysis of human periodontal ligament stem cells by high\u2010resolution mass spectrometry"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1391-236X","authenticated-orcid":false,"given":"Na\u2010Young","family":"Han","sequence":"first","affiliation":[{"name":"Gachon Institute of Pharmaceutical Sciences Gachon College of Pharmacy Gachon University  Incheon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1040-7077","authenticated-orcid":false,"given":"Ji\u2010Youn","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Periodontology School of Dentistry Kyung Hee University  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4407-1383","authenticated-orcid":false,"given":"Jong\u2010Moon","family":"Park","sequence":"additional","affiliation":[{"name":"Gachon Institute of Pharmaceutical Sciences Gachon College of Pharmacy Gachon University  Incheon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changsik","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Penn State University College of Medicine and Milton Hershey Medical Center Hershey  PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saya","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry and Institute of Oral Bioscience Chonbuk National University  Jeonju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0696-8421","authenticated-orcid":false,"given":"Hookeun","family":"Lee","sequence":"additional","affiliation":[{"name":"Gachon Institute of Pharmaceutical Sciences Gachon College of Pharmacy Gachon University  Incheon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3929-4467","authenticated-orcid":false,"given":"Jeong\u2010Ho","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Periodontology College of Dentistry and Institute of Oral Bioscience Chonbuk National University  Jeonju Korea"},{"name":"Research Institute of Clinical Medicine of Chonbuk National University\u2010Biomedical Research Institute of Chonbuk National University Hospital  Jeonju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12477"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00141.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509340867"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00135.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.10.1645"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01174.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2017.02.019"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12378"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01328.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071101"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0446"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/eln031"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-007-1486-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2016.150693"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2015.0017"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2012.01.073"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3028"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-011-0600-5"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/10623329609024704"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2016.03.003"},{"key":"e_1_2_7_23_1","first-page":"548","article-title":"Effect of endoplasmic reticulum stress on the expression and osteogenic differentiation of periodontal ligament stem cells","volume":"50","author":"Xue P","year":"2015","journal-title":"Zhonghua Kou Qiang Yi Xue Za Zhi"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmu099"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.4527"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345980770090501"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00921.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2007.00977.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.13.66"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1947157"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr050109b"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21400"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12604","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T05:50:42Z","timestamp":1694670642000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,8]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/jre.12604"],"URL":"https:\/\/doi.org\/10.1111\/jre.12604","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"type":"print","value":"0022-3484"},{"type":"electronic","value":"1600-0765"}],"subject":[],"published":{"date-parts":[[2018,10,8]]},"assertion":[{"value":"2018-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}