{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:17:07Z","timestamp":1785410227357,"version":"3.56.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81271138"],"award-info":[{"award-number":["81271138"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2017QH007"],"award-info":[{"award-number":["ZR2017QH007"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Periodontal\u00a0ligament\u2010associated protein\u20101 (<jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101) is an important regulator of osteogenic differentiation of\u00a0periodontal\u00a0ligament cells and plays important role in the homeostasis of periodontal tissues. But the role of <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 in periodontitis is poorly understood. Expressions of <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 in experimental periodontitis are observed to elucidate whether <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 gets involved in the pathogenesis of periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Wistar rats were randomly allocated to two groups (n\u00a0=\u00a06\/group): Ligation group and Control group. <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 expression in experimental periodontitis was assessed by immunohistochemistry and collagen fibers in periodontal ligament were observed using picrosirius red staining. Expressions of <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68 in periodontitis were colocalized by double\u2010labelled immunofluorescence. To further examine the relationship between <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 and osteoclastogenesis in experimental periodontitis, acute periodontal inflammatory infiltration and alveolar bone destruction were induced by administering ligated rats with 10\u00a0ng\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> tumor necrosis factor alpha (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1; ligation\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 group, n\u00a0=\u00a06). Alveolar bone loss was observed by micro\u2010computed tomography (Micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>), and osteoclasts were identified by tartrate\u2010resistant acid phosphatase staining (<jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content>). Expressions of <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 in <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 stimulated human periodontal ligament cells were also detected at 24 and 48\u00a0hours by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 expression levels in periodontal ligament cells and collagen fibers were lower in the ligation group,compared with the control group. Similarly, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 decreased <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 expression in human periodontal ligament cells in vitro. Degradation or destruction of collagen fibers accompanied the reduced <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 expression in the periodontal ligament in the ligation group. Colocalization of <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68 revealed\u00a0the positive relationship between <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>68+ infiltrating cells in periodontitis. More <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101\u2010positive inflammatory cells were found in the ligation\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 group, compared with the ligation\u00a0+\u00a0saline group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101\u2010positive inflammatory cells are involved in the pathogenesis of periodontitis. An increase in <jats:styled-content style=\"fixed-case\">PLAP<\/jats:styled-content>\u20101\u2010positive inflammatory cell number contributes periodontal inflammation and alveolar bone loss.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12618","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T06:51:54Z","timestamp":1539067914000},"page":"180-189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Periodontal ligament\u2010associated protein\u20101 gets involved in experimental periodontitis"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9289-5520","authenticated-orcid":false,"given":"Xijiao","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Endodontics Jinan Stomatological Hospital  Jinan China"},{"name":"Shandong Provincial Key Laboratory of Oral Tissue Regeneration School and Hospital of Stomatology Shandong University  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