{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T01:49:46Z","timestamp":1785376186106,"version":"3.55.0"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R00DE022059"],"award-info":[{"award-number":["R00DE022059"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE022032"],"award-info":[{"award-number":["R01DE022032"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R21DE027138"],"award-info":[{"award-number":["R21DE027138"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,6]]},"abstract":"The novel aspect of this study was to contextualize the co\u2010localization of biomolecular expression in widened and narrowed periodontal ligament (PDL)\u2010space within a mechanically activated periodontal complex. The PDL<\/jats:styled-content> is unique as it is the only ligament with both innervation and vascularization. Maxillary molars in 6\u2010week\u2010old male C57BL<\/jats:styled-content>\/6 mice (N\u00a0=\u00a05) were experimentally translated for 2\u00a0weeks using an elastic spacer. Contralateral teeth were used as controls. Mechanical testing of the periodontal complex of a mouse in situ and imaging using X\u2010ray micro\u2010computed tomography (micro\u2010XCT<\/jats:styled-content>) illustrated deformations within blood vessels (BV<\/jats:styled-content>) of the PDL<\/jats:styled-content>. PDL<\/jats:styled-content>\u2010bone and PDL<\/jats:styled-content>\u2010cementum entheses at the widened and narrowed PDL<\/jats:styled-content>\u2010spaces following experimental tooth movement (ETM<\/jats:styled-content>) illustrated osterix (OSX<\/jats:styled-content>), bone sialoprotein (BSP<\/jats:styled-content>), cluster of differentiation 146 (CD<\/jats:styled-content>146), and protein gene product 9.5 (PGP<\/jats:styled-content>9.5), indicating active remodeling at these sites. PGP<\/jats:styled-content>9.5 positive nerve bundles (NB<\/jats:styled-content>s) were co\u2010localized with multinucleated cells (MC<\/jats:styled-content>s), Howship's resorption lacunae, and CD<\/jats:styled-content>146 positive BV<\/jats:styled-content>s. Association between nerves and MC<\/jats:styled-content> was complemented by visualizing the proximity of osmium tetroxide stained NB<\/jats:styled-content>s with the ultrastructure of MC<\/jats:styled-content>s by performing scanning transmission electron microscopy. Spatial association of NB<\/jats:styled-content> with BV<\/jats:styled-content>, and NB<\/jats:styled-content> with MC<\/jats:styled-content>, provided insights into the plausible co\u2010activation of NB<\/jats:styled-content>s to initiate osteoclastic activity. Resorption of mineral occurred as an attempt to restore PDL<\/jats:styled-content>\u2010space of the load\u2010bearing complex, specifically at the PDL<\/jats:styled-content>\u2010entheses. Mapping of anatomy\u2010specific structural elements and their association with regenerative molecules by correlating light and electron micrographs provided insights into the use of these extracellular matrix molecules as plausible targets for pharmacological interventions related to tooth movement. Within the realm of tissue regeneration, modulation of load can reverse naturally occurring mineral formation to experimentally induced resorption, and naturally occurring mineral resorption to experimentally induced formation at the enthesial sites to permit tooth translation.<\/jats:p>","DOI":"10.1111\/jre.12625","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T19:03:30Z","timestamp":1543431810000},"page":"251-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Microanatomical changes and biomolecular expression at the PDL<\/scp>\u2010entheses during experimental tooth movement"],"prefix":"10.1111","volume":"54","author":[{"given":"Lynn","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Biomaterials and Bioengineering Department of Preventive and Restorative Dental Sciences University of California San Francisco 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