{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T04:55:03Z","timestamp":1783140903438,"version":"3.54.6"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T00:00:00Z","timestamp":1547683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:sec><jats:title>Background and objective<\/jats:title><jats:p>The aim of this study was to evaluate the angiogenesis and lymphangiogenesis in gingival tissue biopsy specimens of individuals with clinically healthy gingiva, chronic gingivitis, and chronic periodontitis (n\u00a0=\u00a030 per clinical condition).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Histological sections were stained using hematoxylin and eosin as well as immunohistochemically with hematopoietic progenitor cell antigen <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 and podoplanin (<jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content>) antibodies to evaluate the microvascular count, area, and perimeter of blood and lymphatic vessels, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results revealed a correlation between the microvascular count of blood and lymphatic vessels (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03; however, in individuals with chronic periodontitis, fewer lymphatic vessels were present than in the clinically healthy gingival tissue (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01), which was not observed in the case of microvascular area and perimeter. Podoplanin labeling was present in the epithelium, and the intensity of labeling was positively correlated to the intensity of the inflammatory infiltrate (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.03).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this study, we concluded that an increase in the number of blood and lymphatic vessels was not observed in bouth gingivitis and periodontitis samples. Podoplanin expression is highly associated with an increased inflammatory infiltration suggesting that <jats:styled-content style=\"fixed-case\">PDPN<\/jats:styled-content> might play an additional role in periodontal disease, other than solely as a lymphangiogenesis marker.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12635","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T07:00:21Z","timestamp":1547794821000},"page":"349-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Immunohistochemical study of <scp>CD<\/scp>34 and podoplanin in periodontal disease"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2348-7144","authenticated-orcid":false,"given":"Patr\u00edcia Guerra Peixe","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Department of Dentistry Oral Pathology Federal University of Rio Grande do Norte Natal 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