{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:16:22Z","timestamp":1780586182087,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE012872"],"award-info":[{"award-number":["R01DE012872"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R03DE019805\u201001A1"],"award-info":[{"award-number":["R03DE019805\u201001A1"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1U54 GM104938"],"award-info":[{"award-number":["1U54 GM104938"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,10]]},"abstract":"Objective<\/jats:title>To determine whether circulating levels of two matrix metalloproteinases, MMP<\/jats:styled-content>\u20102 and MMP<\/jats:styled-content>\u20109, are associated with loss of alveolar bone density (ABD<\/jats:styled-content>) or height (ABH<\/jats:styled-content>), or with progression of periodontitis (relative clinical attachment level [RCAL<\/jats:styled-content>]), among postmenopausal women with local and systemic bone loss.<\/jats:p><\/jats:sec>Background<\/jats:title>This study was planned as part of a 2\u2010year randomized, double\u2010blind, placebo\u2010controlled, clinical trial examining efficacy\/safety of subantimicrobial dose doxycycline (20\u00a0mg bid) in postmenopausal osteopenic women. This study examines whether serum levels of gelatinases are associated with local changes in the periodontium.<\/jats:p><\/jats:sec>Methods<\/jats:title>A sample of 113 women received periodontal maintenance for moderate to advanced chronic periodontitis and consented to analysis of stored serum biomarkers. Posterior vertical bitewings were taken, and serum collected, at baseline, one, and 2\u00a0years. ABD<\/jats:styled-content> was determined by computer\u2010assisted densitometric image analysis (CADIA<\/jats:styled-content>), ABH<\/jats:styled-content> by the Hausmann et\u00a0al (1992, J Periodontol<\/jats:italic> 63, 657) method, and RCAL<\/jats:styled-content> by Florida Probe (every 6\u00a0months). MMP<\/jats:styled-content>s were measured densitometrically on gelatin zymograms using denatured type I collagen as substrate and purified MMP<\/jats:styled-content>\u20102 (72\u00a0kDa) and MMP<\/jats:styled-content>\u20109 (92\u00a0kDa) as standards. Evidence of worsening in the periodontium at a tooth site was defined as a change from baseline of, for ABD<\/jats:styled-content>, at least 14 densitometric units (for subcrestal locations) or 17 units (for crestal locations); of at least 0.4\u00a0mm for ABH<\/jats:styled-content>; and of at least 1.5\u00a0mm for RCAL<\/jats:styled-content>. Logistic regression models, while accounting for clustering, compared the odds of worsening in ABD<\/jats:styled-content>, ABH<\/jats:styled-content>, or RCAL<\/jats:styled-content>, after 2\u00a0years of observation, between groups defined by baseline and concurrent levels of serum gelatinases.<\/jats:p><\/jats:sec>Results<\/jats:title>Changes in ABH<\/jats:styled-content> and RCAL<\/jats:styled-content> were not associated with circulating levels of MMP<\/jats:styled-content>\u20102 or MMP<\/jats:styled-content>\u20109. However, elevated odds of ABD<\/jats:styled-content> loss over 24\u00a0months were associated, among smokers, with both baseline and concurrent levels of MMP<\/jats:styled-content>\u20109 in the middle and highest tertile, and with concurrent levels of MMP<\/jats:styled-content>\u20102 in the middle (but not the highest) tertile. Elevated odds of ABD<\/jats:styled-content> loss were also associated, among women within 5\u00a0years of menopause, with baseline levels of MMP<\/jats:styled-content>\u20102 in the highest tertile.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Among postmenopausal osteopenic women, loss of ABD<\/jats:styled-content> was associated, in smokers, with elevated circulating levels of MMP<\/jats:styled-content>\u20109 and MMP<\/jats:styled-content>\u20102. In those within 5\u00a0years of menopause, ABD<\/jats:styled-content> loss was associated with elevated circulating levels of MMP<\/jats:styled-content>\u20102.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12656","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T10:51:29Z","timestamp":1556535089000},"page":"525-532","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Loss of alveolar bone density in postmenopausal, osteopenic women is associated with circulating levels of gelatinases"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8934-4181","authenticated-orcid":false,"given":"David M.","family":"Thompson","sequence":"first","affiliation":[{"name":"Department of Biostatistics and Epidemiology Hudson College of Public Health University of Oklahoma Health Sciences Center Oklahoma City 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