{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T14:09:48Z","timestamp":1766066988727,"version":"3.40.5"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T00:00:00Z","timestamp":1555286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017R1A5A2015391","NRF-2014R1A1A3053711","NRF-2015R1C1A2A01055438","NRF-2017M3A9E4047243"],"award-info":[{"award-number":["NRF-2017R1A5A2015391","NRF-2014R1A1A3053711","NRF-2015R1C1A2A01055438","NRF-2017M3A9E4047243"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>We evaluated the role of oleanolic acid acetate (OAA), a triterpenoid commonly used in the treatment of liver disorders, inflammatory diseases, and metastasis, in bone formation after tooth loss by periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Periodontitis causes the sequential degradation of the alveolar bone and associated structures, resulting in tooth loss. Several studies have attempted to regenerate the bone for implantation following tooth loss.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Maxillary left second molar was extracted from 8\u2010week\u2010old male mice following induction of periodontitis by ligature for 5\u00a0days. The extraction socket was treated with 50\u00a0ng\/\u00b5L OAA for 1, 2, and 3\u00a0weeks. Detailed morphological changes were examined using Masson's trichrome staining, and the precise localization patterns of various signaling molecules, including CD31, F4\/80, interleukin (IL)\u20106, and osteocalcin, were observed. The volume of bone formation was examined by Micro\u2010CT. Osteoclasts were enumerated using tartrate\u2010resistant acid phosphatase (TRAP) staining. For molecular dissection of signaling molecules, we employed the hanging\u2010drop in vitro cultivation method at E14 for 1\u00a0day and examined the expression pattern of transforming growth factor (TGF)\u2010\u03b2 superfamily and Wnt signaling genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histomorphometrical examinations showed facilitated bone formation in the extraction socket following OAA treatment. In addition, OAA\u2010treated specimens showed the altered localization patterns of inflammatory and bone formation\u2010related signaling molecules including CD31, F4\/80, IL\u20106, and osteocalcin. Also, embryonic tooth germ mesenchymal tissue cultivation with OAA treatment showed the significant altered expression patterns of signaling molecules such as transforming growth factor (TGF)\u2010\u03b2 superfamily and Wnt signaling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Oleanolic acid acetate induces bone formation and remodeling through proper modulation of osteoblast, osteoclast, and inflammation with regulations of TGF\u2010\u03b2 and Wnt signaling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12657","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T07:50:02Z","timestamp":1555314602000},"page":"533-545","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice"],"prefix":"10.1111","volume":"54","author":[{"given":"Nirpesh","family":"Adhikari","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Dentistry IHBR, Kyungpook National University Daegu Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjiv","family":"Neupane","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry IHBR, Kyungpook National University Daegu Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yam Prasad","family":"Aryal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry IHBR, Kyungpook National University Daegu Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Radiology, School of Dentistry IHBR, Kyungpook National University Daegu 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Korea Research Institute of Bioscience and Biotechnology Jeongeup\u2010si Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae\u2010Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry Chonnam National University Gwangju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Histology and Developmental Biology Tokyo Dental College Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Hyeon","family":"An","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Radiology, School of Dentistry IHBR, Kyungpook National University Daegu 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