{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:55:18Z","timestamp":1791888918080,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,4,14]],"date-time":"2019-04-14T00:00:00Z","timestamp":1555200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011142","name":"Graduate School, University of Minnesota","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011142","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Wnt signaling pathways regulate osteoblast differentiation and bone formation and are associated with inflammatory responses driven by innate and adaptive immunity via the NF\u2010\u03baB pathway. The aim of this study was to compare the levels of sclerostin (SOST), WNT\u20105a, and TNF\u2010\u03b1 between chronic periodontitis and periodontally healthy sites and determine their value as diagnostic markers of chronic periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>In a cross\u2010sectional assessment 25 chronic periodontitis cases and 25 periodontally healthy controls were selected upon clinical and radiographic periodontal evaluation. Gingival crevicular fluid (GCF) was collected cross\u2010sectionally from diseased and healthy sites in periodontitis patients and from healthy sites in each control subject. In a subgroup analysis, ten patients with generalized moderate and severe chronic periodontitis and ten generalized periodontally healthy individuals were included. The protein levels of SOST, WNT\u20105a, and TNF\u2010\u03b1 in GCF were measured by sandwich ELISA. The Shapiro\u2010Wilk test was utilized to assess the normality of the distribution and non\u2010parametric comparisons were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The protein levels of SOST were significantly higher in the generalized moderate and severe chronic periodontitis subgroup when compared to the generalized healthy (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.002), while the WNT\u20105a and TNF\u2010\u03b1 GCF total amounts were similar (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05). Diseased sites in the periodontitis patients exhibited significantly higher total protein levels of WNT\u20105a than in healthy sites (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.017), whereas no differences were detected for SOST and TNF\u2010\u03b1 (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05). The total protein levels of SOST, WNT\u20105a, and TNF\u2010\u03b1 in GCF were similar in periodontitis and non\u2010periodontitis patients (<jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Sclerostin and WNT\u20105a gingival protein levels demonstrated a high diagnostic value for generalized moderate and severe chronic periodontitis, while a low accuracy was detected for localized chronic periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12659","type":"journal-article","created":{"date-parts":[[2019,4,15]],"date-time":"2019-04-15T06:34:56Z","timestamp":1555310096000},"page":"555-565","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Sclerostin and WNT\u20105a gingival protein levels in chronic periodontitis and health"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6951-8794","authenticated-orcid":false,"given":"Georgios S.","family":"Chatzopoulos","sequence":"first","affiliation":[{"name":"Division of Periodontology, Department of Developmental and Surgical Sciences, School of Dentistry University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim C.","family":"Mansky","sequence":"additional","affiliation":[{"name":"Division of Orthodontics, Department of Developmental and Surgical Sciences, School of Dentistry University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Lunos","sequence":"additional","affiliation":[{"name":"Biostatistical Design and Analysis Center, Clinical and Translational Science Institute University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Costalonga","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Developmental and Surgical Sciences, School of Dentistry University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry F.","family":"Wolff","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Developmental and Surgical Sciences, School of Dentistry University of Minnesota Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,4,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0089-x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v3i0.5304"},{"key":"e_1_2_7_4_1","first-page":"1325","article-title":"Interaction between osteoblast and osteoclast: impact in bone disease","volume":"19","author":"Phan TC","year":"2004","journal-title":"Histol Histopathol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-010-1272-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001090100226"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.12.8536"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2009.01.003"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28551"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509338340"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-013-9749-z"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413274200"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025900"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.081206"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.182"},{"key":"e_1_2_7_16_1","first-page":"731","article-title":"WNT5A expression in human breast cancer","volume":"25","author":"Leris A","year":"2005","journal-title":"Anticancer Res"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2653"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2419"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.050574297"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2014.08.027"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2015.06.010"},{"key":"e_1_2_7_22_1","first-page":"470","article-title":"Expression of Wnt5a in chronic apical periodontitis and its clinical significance","volume":"24","author":"Xu LY","year":"2015","journal-title":"Shanghai Kou Qiang Yi Xue"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt065"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.1.403"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03260.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.06.100"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034512457373"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01670.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2003.03106.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb01106.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1997.2.1.75"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1973.tb00773.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1990.tb00910.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1989.tb01650.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1997.2.1.123"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1985.56.11s.13"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb01129.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1992.tb01819.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2003.03104.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12245"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2015.150270"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034434"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516687248"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22010"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-008-9069-9"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13192"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032437"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2014.1724"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9290.102221"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb01649.x"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070422"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(90)90205-O"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0039-1698345"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12659","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jre.12659","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12659","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T13:52:08Z","timestamp":1694267528000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12659"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,14]]},"references-count":53,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,10]]}},"alternative-id":["10.1111\/jre.12659"],"URL":"https:\/\/doi.org\/10.1111\/jre.12659","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,14]]},"assertion":[{"value":"2018-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}