{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:55:52Z","timestamp":1780988152685,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T00:00:00Z","timestamp":1566777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Perforated barrier membranes (PBM) were suggested to enhance periodontal regeneration by allowing positive charity of wanted elements from the gingival tissue side. The present study was designed to evaluate clinically and biochemically the use of PBM combined with simvastatin (SMV) gel with and without an associated EDTA gel root surface etching as a suggested option that could improve SMV availability and clinical outcomes of PBM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty patients having moderate\u2010to\u2010severe chronic periodontitis with 40 intrabony defects were randomly divided into four treatment groups (10 sites each). Patients in group 1 received 1.2% SMV gel and covering the defect with occlusive membrane (OM). Patients in group 2 received 1.2% SMV gel and covering the defect with PBM. Group 3 received 24% EDTA root surface etching, 1.2% SMV gel, and defect coverage with OM (eOM). Patients in group 4 were treated as in group 3 but the defect was covered with PBM (ePBM). Clinical parameters were recorded at baseline before surgical procedures and were reassessed at 6 and 9\u00a0months after therapy. The mean concentration of SMV in gingival crevicular fluid (GCF) was estimated by reverse\u2010phase high\u2010performance liquid chromatography at days 1, 7, 14, 21, and 30.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 6\u2010 and 9\u2010month observation periods, groups 3 and 4 showed a statistically significant improvement in PD reduction and CAL gain compared with groups 1 and 2. Group 4 showed a statistically significant more defect fill compared with groups 1, 2, and 3 (<jats:italic>P<\/jats:italic>\u00a0\u2264\u00a0.05). Group 2 showed statistically significant higher defect fill compared with group 1 and group 3 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Bone density was significantly increased with no significant difference between the four groups at 6\u2010 and 9\u2010month observation periods. SMV\u2010GCF concentration in group 4 showed the highest mean concentration with no significant difference than that of group 3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The use of perforated barrier membranes in association with SMV enhances the clinical hard tissue parameters compared with occlusive ones in treating intrabony periodontal defects. Moreover, EDTA root surface treatment could enhance SMV availability in the defect area.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12692","type":"journal-article","created":{"date-parts":[[2019,8,26]],"date-time":"2019-08-26T11:49:50Z","timestamp":1566820190000},"page":"85-95","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Guided tissue regeneration of intrabony defects with perforated barrier membranes, simvastatin, and EDTA root surface modification: A clinical and biochemical study"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7643-4591","authenticated-orcid":false,"given":"Dalia Rasheed","family":"Issa","sequence":"first","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Faculty of Dentistry Kafrelsheikh University Kafr El Sheikh Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaled A.","family":"Abdel\u2010Ghaffar","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dental Medicine Ain Shams University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Al\u2010Shahat","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dental Medicine Delta University for science and technology Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Abdel Aziz","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Dental Medicine Ain Shams University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent J.","family":"Iacono","sequence":"additional","affiliation":[{"name":"School of Dental Medicine Stony Brook University Stony Brook NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3032-4896","authenticated-orcid":false,"given":"Ahmed Y.","family":"Gamal","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Faculty of Oral and Dental Medicine Nahda University Benisweif Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2011.00432.x"},{"key":"e_1_2_9_3_1","first-page":"76","article-title":"Clinical re\u2010entry and histologic evaluation of periodontal intrabony defects following the use of marginal periosteal pedicle graft as an autogenous guided tissue membrane","volume":"12","author":"Gamal AY","year":"2010","journal-title":"J Int Acad Periodontol"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.100277"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.120301"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902318"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00543.x"},{"key":"e_1_2_9_8_1","first-page":"1","article-title":"Gingival crevicular fluid bone morphogenetic protein\u20102 release profile following the use of modified perforated membrane barriers in localized intrabony defects. 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