{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T08:30:16Z","timestamp":1748421016201,"version":"3.40.5"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:00:00Z","timestamp":1569369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["F30DE027210","R01DE021134"],"award-info":[{"award-number":["F30DE027210","R01DE021134"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Periodontal disease (PD) afflicts approximately 50% of the population in the United States and is characterized by chronic inflammation of the periodontium that can lead to loss of the periodontal ligament through collagen degradation, loss of alveolar bone, and to eventual tooth loss. Previous studies have implicated transglutaminase (TG) activity in promoting thin collagen I fiber morphology and decreased mechanical strength in homeostatic PDL. The aim of this study was to determine whether TG activity influenced collagen assembly in PDL in the setting of periodontal disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>A ligature model was used to induce clinically relevant PD in mice. Mice with ligature were assessed at 5 and 14\u00a0days to determine PDL collagen morphology, transglutaminase (TG) activity, and bone loss. The effects of inhibition of TG on PDL were assessed by immunohistochemistry and second\u2010harmonic generation (SHG) to visualize collagen fibers in native tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ligature placement around the 2nd molar resulted in significant bone loss and a decrease in total collagen content after 5\u00a0days of ligature placement. A significant increase in thin over thick fibers was also demonstrated in mice with ligature at 5\u00a0days associated with apparent increases in immunoreactivity for TG2 and for TG\u2010mediated N\u2010\u03b5\u2010\u03b3\u2010glutamyl cross\u2010links in PDL. Inhibition of TG activity increased total collagen and thick collagen fiber content over vehicle control in mice with ligature for 5\u00a0days. SHG of PDL was used to visualize and quantify the effects of TG inhibition on enhanced collagen fiber organization in unfixed control and diseased PDL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These studies support a role of TG in regulating collagen fiber assembly and suggest that strategies to inhibit TG activity in disease might contribute to restoration of PDL tissue integrity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12694","type":"journal-article","created":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T18:56:38Z","timestamp":1569437798000},"page":"107-115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Inhibition of transglutaminase activity in periodontitis rescues periodontal ligament collagen content and architecture"],"prefix":"10.1111","volume":"55","author":[{"given":"Emilie","family":"Moore Rosset","sequence":"first","affiliation":[{"name":"Department of Oral Health Sciences Medical University of South Carolina Charleston SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Trombetta\u2010eSilva","sequence":"additional","affiliation":[{"name":"Department of Oral Health Sciences Medical University of South Carolina Charleston SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn","family":"Hepfer","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Clemson University Clemson SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Clemson University Clemson SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Oral Health Sciences Medical University of South Carolina Charleston SC USA"},{"name":"Department of Bioengineering Clemson University Clemson SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9202-3044","authenticated-orcid":false,"given":"Amy D.","family":"Bradshaw","sequence":"additional","affiliation":[{"name":"Department of Oral Health Sciences Medical University of South Carolina Charleston SC USA"},{"name":"Division of Cardiology, Department of Medicine Gazes Cardiac Research Institute, Medical University of South Carolina Charleston SC USA"},{"name":"Ralph H. Johnson Department of Veterans Affairs Medical Center Charleston SC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00094.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2011.100710"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.53.070184.003441"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-008-0190-y"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.16.2989"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.148.4.825"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2522"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.05.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510391800"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-013-0939-7"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.004868"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000329675"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02084-14"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2741\/2030"},{"key":"e_1_2_8_16_1","first-page":"1399","article-title":"Transglutaminase 2 expression is significantly increased in cyclosporine\u2010induced gingival overgrowth","volume":"26","author":"Asioli S","year":"2011","journal-title":"Histol Histopathol"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.11607\/ijp.4280"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12180"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2015.08.004"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-014-1864-2"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12694","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jre.12694","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T20:23:28Z","timestamp":1694118208000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12694"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,25]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/jre.12694"],"URL":"https:\/\/doi.org\/10.1111\/jre.12694","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"type":"print","value":"0022-3484"},{"type":"electronic","value":"1600-0765"}],"subject":[],"published":{"date-parts":[[2019,9,25]]},"assertion":[{"value":"2019-01-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}