{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:06:30Z","timestamp":1789405590941,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,20]],"date-time":"2019-09-20T00:00:00Z","timestamp":1568937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and objectives<\/jats:title>\n                    <jats:p>Strontium ranelate is a medication indicated for the treatment of osteoporosis that presents concomitant anti\u2010resorptive and osteoanabolic dual biological activity. However, the effects of strontium ranelate on alveolar bone have been poorly explored. Furthermore, to date, there are no data on the effects of this medication on alveolar bone loss (BL) during conditions of estrogen deficiency. Therefore, the aim of this study was to evaluate the effects of strontium ranelate on ligature\u2010induced periodontitis in estrogen\u2010deficient and estrogen\u2010sufficient rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ninety\u2010six rats were assigned to one of the following groups: sham\u2010surgery\u00a0+\u00a0water (estrogen\u2010sufficient; n\u00a0=\u00a024); ovariectomy\u00a0+\u00a0water (estrogen\u2010deficient; n\u00a0=\u00a024), sham\u2010surgery\u00a0+\u00a0strontium ranelate (ranelate\/estrogen\u2010sufficient; n\u00a0=\u00a024) and; ovariectomy\u00a0+\u00a0strontium ranelate (ranelate\/estrogen\u2010deficient; n\u00a0=\u00a024). The rats received strontium ranelate or water from the 14th day after ovariectomy until the end of the experiment. On the 21st day after ovariectomy, one first mandibular molar received a ligature, while the contralateral tooth was left unligated. Eight rats per group were killed at 10, 20, and 30\u00a0days after ligature placement. Bone loss (BL) and trabecular bone area (TBA) were analyzed in the furcation area of ligated and unligated teeth at all experimental times by histometry. Tartrate\u2010resistant acid phosphatase (TRAP) positive cells and immunohistochemical staining for osteocalcin (OCN), osteopontin (OPN), osteoprotegerin (OPG), and receptor activator of NF\u2010\u041aB ligand (RANKL) were assessed in the ligated teeth at 30\u00a0days after ligature placement.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At 10 and 30\u00a0days, ligated teeth of the estrogen\u2010deficient group exhibited higher BL, when compared to all other groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). At 10\u00a0days, TBAs were higher in the unligated teeth of strontium ranelate\u2010treated groups, when compared to those of untreated groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). At 30\u00a0days, the ligated teeth of the estrogen\u2010deficient group exhibited lower TBA than the other groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). There were no differences among groups regarding the number of TRAP\u2010stained cells (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). The strontium ranelate\u2010treated groups exhibited lower expressions of OCN and RANKL than the untreated groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05). The estrogen\u2010sufficient group presented higher staining for OPG than both treated and untreated estrogen\u2010deficient groups (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Strontium ranelate prevented ligature\u2010induced BL in an estrogen\u2010deficiency condition and, to a certain extent, increased TBA in the presence and absence of periodontal collapse in states of estrogen deficiency and estrogen sufficiency. Furthermore, strontium ranelate also affected the expression of bone markers, appearing to have acted predominantly as an anti\u2010resorptive agent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.12697","type":"journal-article","created":{"date-parts":[[2019,9,20]],"date-time":"2019-09-20T09:13:10Z","timestamp":1568970790000},"page":"141-151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of strontium ranelate on ligature\u2010induced periodontitis in estrogen\u2010deficient and estrogen\u2010sufficient rats"],"prefix":"10.1111","volume":"55","author":[{"given":"Let\u00edcia Macedo","family":"Marins","sequence":"first","affiliation":[{"name":"Dental Research Division Department of Periodontology Guarulhos University Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo 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