{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:21:52Z","timestamp":1780939312494,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T00:00:00Z","timestamp":1569369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81670990"],"award-info":[{"award-number":["81670990"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>It has been reported that long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s), such as metastasis\u2010associated lung adenocarcinoma transcript 1 (<jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1), act as key regulators of the development of inflammatory diseases. However, it is unclear whether <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 regulates the function of human gingival fibroblasts (<jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>s) in periodontitis. This study is to explore the role of <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 on inflammatory cytokine production of <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Primary <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>s were harvested from human gingiva. <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 was detected in inflammatory and healthy gingival tissues via quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Bioinformatics analysis, dual\u2010luciferase reporter assay, and <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010binding protein immunoprecipitation (<jats:styled-content style=\"fixed-case\">RIP<\/jats:styled-content>) were used to detect the relationship among <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1, toll\u2010like receptor 4 (<jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4), and micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (miR) \u201020a. After transfection <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010treated <jats:styled-content style=\"fixed-case\">HGF<\/jats:styled-content>s with <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (si\u2010<jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1), miR\u201020a mimic or overexpression <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 plasmid (sno\u2010<jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1), the levels of <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1, miR\u201020a, <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 were analyzed by <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, enzyme\u2010linked immunosorbent assay, or western blot assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 up\u2010regulated in inflammatory gingival tissues of chronic periodontitis. MiR\u201020a was bound with <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4 3\u2032\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010protein complex with Ago2, respectively. Moreover, <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 increased while miR\u201020a decreased after 1\u00a0\u03bcg\/mL <jats:italic>Porphyromonas gingivalis<\/jats:italic> lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>) or <jats:italic>Escherichia coli <\/jats:italic><jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> stimulation. MiR\u201020a inhibited the expression of proinflammatory cytokines via binding to <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4 3\u2032\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content>. In addition, <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 increased <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4 level and the secretion of inflammatory cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 enhances inflammatory cytokine production through sponging miR\u201020a and releasing <jats:styled-content style=\"fixed-case\">TLR<\/jats:styled-content>4, indicating a regulatory role of <jats:styled-content style=\"fixed-case\">MALAT<\/jats:styled-content>1 in periodontal inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12700","type":"journal-article","created":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T18:53:20Z","timestamp":1569437600000},"page":"182-190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Lnc<scp>RNA MALAT<\/scp>1 regulates inflammatory cytokine production in lipopolysaccharide\u2010stimulated human gingival fibroblasts through sponging miR\u201020a and activating <scp>TLR<\/scp>4 pathway"],"prefix":"10.1111","volume":"55","author":[{"given":"Jiashan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Periodontics Hospital of Stomatology School of Dentistry Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minwei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biological Science University of California Irvine Irvine CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liting","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Periodontics Hospital of Stomatology School of Dentistry Tianjin Medical University Tianjin 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