{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:47:30Z","timestamp":1790858850682,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2013\/08589\u20103"],"award-info":[{"award-number":["2013\/08589\u20103"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/02713\u20102"],"award-info":[{"award-number":["2016\/02713\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/11419\u20100"],"award-info":[{"award-number":["2016\/11419\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Some studies suggest that regulatory T cells (Tregs) have suppressive effects on inflammatory osteolysis. The aim of this study was to evaluate Treg immunomarkers in periodontitis\u2010affected tissues from patients with periodontitis and clinically healthy gingiva (control).<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The presence and distribution of positive cells for CD4, CD25 and FOXP3 (Treg immunomarkers) in periodontitis\u2010affected tissues (epithelium and lamina propria) of 30 patients (ten per group) with a diagnosis of stage IV, grade C periodontitis (IV\u2010C), stage III, grade B periodontitis (III\u2010B) and the control were evaluated. A two\u2010way ANOVA followed by Fisher's LSD test was used to demonstrate differences between the groups and immunomarkers; Student's t test was used to demonstrate differences between the epithelium and the lamina propria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both IV\u2010C and III\u2010B periodontitis presented a significantly high proportion of immune\u2010stained cells for all immunomarkers when compared to the control group. Notably, CD25+ and FOXP3+ cells were detected in a significantly higher number in III\u2010B than IV\u2010C periodontitis (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest the participation of Tregs on the osteoimmunological mechanisms in IV\u2010C and III\u2010B periodontitis patients, notably contributing to strategies for alveolar bone regeneration in clinical treatment decisions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12721","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T14:16:22Z","timestamp":1577369782000},"page":"374-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["FOXP3+ and CD25+ cells are reduced in patients with stage IV, grade C periodontitis: A comparative clinical study"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9149-1831","authenticated-orcid":false,"given":"Raphael J. G.","family":"da Motta","sequence":"first","affiliation":[{"name":"Integrated Dental Clinic Department of Dental Materials and Prosthodontics School of Dentistry of Ribeir\u00e3o Preto (FORP\/USP) University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-4052","authenticated-orcid":false,"given":"Luciana Yamamoto","family":"Almeida","sequence":"additional","affiliation":[{"name":"Haematology Division Department of Clinical Medicine Ribeir\u00e3o Preto Medical School (FMRP\/USP) University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0231-5482","authenticated-orcid":false,"given":"Kelly R. V.","family":"Villafuerte","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeir\u00e3o Preto (FORP\/USP) University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3579-5048","authenticated-orcid":false,"given":"Alfredo","family":"Ribeiro-Silva","sequence":"additional","affiliation":[{"name":"Department of Pathology Ribeir\u00e3o Preto Medical School (FMRP\/USP) University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9668-5870","authenticated-orcid":false,"given":"Jorge E.","family":"Le\u00f3n","sequence":"additional","affiliation":[{"name":"Oral Pathology Department of Stomatology Public Oral Health, and Forensic Dentistry School of Dentistry of Ribeir\u00e3o Preto (FORP\/USP) University of S\u00e3o Paulo 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