{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T02:29:34Z","timestamp":1784946574800,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T00:00:00Z","timestamp":1579478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500898"],"award-info":[{"award-number":["81500898"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study investigated the effects of poly lactic\u2010co\u2010glycolic acid (PLGA) loaded with plasmid DNA encoding fibroblast growth factor\u20102 (pFGF\u20102) on human periodontal ligament cells (hPDLCs) in vitro and evaluated the ability of the PLGA\/pFGF\u20102 scaffold to promote periodontal ligament (PDL) regeneration in a beagle dog teeth avulsion animal model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Growth factor and scaffold play important roles in PDL regeneration. PLGA is a kind of biodegradable and biocompatible polymer that can be used as a carrier to deliver growth factors or genes. FGF\u20102 can induce potent proliferative responses, promote cell migration and regulate the production of extracellular matrix. Therefore, a gene\u2010activated matrix composed of scaffold and genes is supposed to be a superior approach for promoting tissue regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, PLGA and PLGA\/pFGF\u20102 scaffolds were fabricated using electrospinning. The characterization of scaffolds was shown by scanning electron microscope (SEM) and transmission electron microscope (TEM). dsDNA HS was used to test the plasmid release of PLGA\/pFGF\u20102 scaffold. The viability and proliferation of hPDLCs on the two kinds of scaffolds were evaluated by the CCK\u20108 assay, and the expression of <jats:italic>collagen I<\/jats:italic> and <jats:italic>scleraxis<\/jats:italic> were analysed by RT\u2010qPCR. The roots of avulsed teeth were covered by the two types of scaffolds and replanted into the alveolar pockets in beagles. Haematoxylin\u2010eosin and Masson staining were used to evaluate the effects of PLGA\/pFGF\u20102 scaffold on promoting PDL regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The smooth and uniform fibres can be observed in both scaffolds, and the plasmids were randomly distributed in the PLGA\/pFGF\u20102 scaffold. dsDNA HS analysis demonstrated that the PLGA\/pFGF\u20102 scaffold released up to 123\u00a0ng pFGF\u20102 over 21\u00a0days in a sustained manner without any obvious burst release. The PLGA\/pFGF\u20102 scaffold promoted the proliferation of hPDLCs and increased the expression levels of <jats:italic>collagen I<\/jats:italic> and <jats:italic>scleraxis<\/jats:italic> compared with PLGA scaffold. Animal experiments showed that more regular PDL\u2010like tissues and less root surface resorption occurred in the PLGA\/pFGF\u20102 scaffold group compared with the PLGA scaffold group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The PLGA\/pFGF\u20102 scaffold promoted hPDLCs proliferation and facilitated periodontal ligament\u2010related differentiation<jats:italic>.<\/jats:italic> The PLGA\/pFGF\u20102 scaffold possesses excellent biological characteristics and could be used as a promising biomaterial for improving the treatment prognosis of replanted tooth.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12734","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T06:35:56Z","timestamp":1579588556000},"page":"488-495","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Poly lactic\u2010co\u2010glycolic acid scaffold loaded with plasmid DNA encoding fibroblast growth factor\u20102 promotes periodontal ligament regeneration of replanted teeth"],"prefix":"10.1111","volume":"55","author":[{"given":"Liming","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Paediatric Dentistry School of Stomatology China Medical University Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenjiang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Paediatric Dentistry School of Stomatology China Medical University Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Paediatric Dentistry School of Stomatology China Medical University Shenyang Liaoning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Paediatric Dentistry School of Stomatology 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