{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T17:34:19Z","timestamp":1774546459224,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,4,22]],"date-time":"2020-04-22T00:00:00Z","timestamp":1587513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81870791"],"award-info":[{"award-number":["81870791"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Though impacts of traumatic occlusion (TO) on periodontal tissues and roles of cystathionine <jats:italic>\u03b3<\/jats:italic>\u2010lyase (<jats:italic>Cth<\/jats:italic>) gene in the regulation of bone homeostasis have been studied by many, no consensus has been reached so far on whether TO deteriorates the periodontium and precise roles of <jats:italic>Cth<\/jats:italic> in occlusal trauma. Therefore, this study aims to investigate the impacts of TO on periodontal tissues and the involvement of <jats:italic>Cth<\/jats:italic> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighty C57BL\/6 wild\u2010type (WT) mice and <jats:italic>Cth<\/jats:italic> knockout (<jats:italic>Cth<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>) mice, 8\u00a0weeks old, were used in this study. The TO model was established using composite resin bonding on the left maxillary molar for one, two, and three weeks, respectively. Morphological and histological changes in the periodontium were assessed by micro\u2010computed tomography (micro\u2010CT), hematoxylin and eosin (H&amp;E) staining, and tartrate\u2010resistant acid phosphatase (TRAP) staining. Osteoclast\u2010related genes were analyzed by real\u2010time polymerase chain reaction (qPCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>It was found that decreased alveolar bone height, expanded bone resorption area, and increased width of periodontal ligament (PDL) occurred in TO models, accompanied by an increased number of osteoclasts in a time\u2010dependent manner by micro\u2010CT and histological staining. Osteoclast\u2010related genes including <jats:italic>Ctsk<\/jats:italic>, <jats:italic>Mmp9<\/jats:italic>, <jats:italic>Rank<\/jats:italic>, <jats:italic>Trap,<\/jats:italic> and <jats:italic>Rankl\/Opg<\/jats:italic> were also up\u2010regulated after one week of modeling. The up\u2010regulated expressions of <jats:italic>Cth<\/jats:italic> gene and its protein CTH were observed in TO mouse models. After 1, 2, or 3\u00a0weeks of modeling, WT mice showed more severe alveolar bone resorption, wider PDL, higher osteoclast count, and higher levels of osteoclast\u2010related genes <jats:italic>Ctsk<\/jats:italic>, <jats:italic>Rank<\/jats:italic>, and <jats:italic>Rankl\/Opg<\/jats:italic> than <jats:italic>Cth<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TO causes a reduction in alveolar bone height and PDL morphological disorder with their severity increases in a time\u2010dependent manner. <jats:italic>Cth<\/jats:italic> aggravates periodontal damage caused by TO.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12753","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T02:50:23Z","timestamp":1587610223000},"page":"667-675","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cystathionine gamma\u2010lyase aggravates periodontal damage in traumatic occlusion mouse models"],"prefix":"10.1111","volume":"55","author":[{"given":"Liyuan","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthodontics School of Stomatology Shanghai Engineering Research Center of Tooth Restoration and Regeneration Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caizhu","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Shanghai Engineering Research Center of Tooth Restoration and Regeneration Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7860-9375","authenticated-orcid":false,"given":"Yongmei","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Orthodontics School of Stomatology Shanghai Engineering Research Center of Tooth Restoration and Regeneration Tongji University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00402-012-1558-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110514"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12109"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345730520054401"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1995.tb00260.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345710500020501"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.03.005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-004-0072-x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2016.6826"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517690388"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.05.015"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2015.150240"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00773.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2008.00545.x"},{"issue":"2","key":"e_1_2_8_16_1","first-page":"354","article-title":"RANKL\/RANK\/OPG molecular complex\u2013control factors in bone remodeling in psoriatic arthritis","volume":"115","author":"Ciacli C","year":"2011","journal-title":"Rev Med Chir Soc Med Nat Iasi"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80209-3"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.23.3037"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13065"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00514.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02408.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12251"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9290.199600"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1976.tb00082.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1974.tb00661.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1968.39.2.101"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1976.47.2.63"},{"issue":"3","key":"e_1_2_8_28_1","first-page":"79","article-title":"Periodontal disease and occlusal trauma: a still debated controversy? 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