{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T13:53:33Z","timestamp":1784728413420,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective and background<\/jats:title><jats:p>Little is known about structural and mechanical properties of leukocyte\u2010 and platelet\u2010rich fibrin (L\u2010PRF) membranes and even less about the influence of antithrombotic drugs on L\u2010PRF. The aim of this in vitro study is therefore to investigate mechanical properties, fibrin structure and cell content of L\u2010PRF membranes and the impact of anticoagulant therapy on L\u2010PRF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples were obtained from 12 volunteers and supplemented with either no, 1.25\u00a0IU, 2.5\u00a0IU, 5\u00a0IU, or 10\u00a0IU enoxaparin. L\u2010PRF membranes were characterized with tensile testing, scanning electron microscopy, and measurement of platelets and leukocytes. Control and enoxaparin\u2010supplemented L\u2010PRF membranes were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At 10\u00a0IU enoxaparin, no L\u2010PRF membranes could be generated, whereas the low doses of 1.25 and 2.5\u00a0IU had no influence on L\u2010PRF properties. The mechanical properties, fibrin networks, and number of platelets and leukocytes of 5\u00a0IU supplemented membranes were unlike the control membranes, but were not found to be significantly different because of limited sampling and inter\u2010 and intra\u2010variability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Low doses of the anticoagulant enoxaparin do not affect mechanical properties, fibrin network, nor cellular content of L\u2010PRF, whereas high doses impair L\u2010PRF generation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12755","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T09:46:06Z","timestamp":1589449566000},"page":"686-693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Mechanical and structural properties of leukocyte\u2010 and platelet\u2010rich fibrin membranes: An in vitro study on the impact of anticoagulant therapy"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0635-2774","authenticated-orcid":false,"given":"Anna","family":"Ockerman","sequence":"first","affiliation":[{"name":"OMFS IMPATH Research Group Faculty of Medicine Department of Imaging and Pathology University of Leuven Leuven Belgium"},{"name":"Oral and Maxillofacial Surgery University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel","family":"Braem","sequence":"additional","affiliation":[{"name":"Surface and Interface Engineered Materials Department of Materials Engineering University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"EzEldeen","sequence":"additional","affiliation":[{"name":"OMFS IMPATH Research Group Faculty of Medicine Department of Imaging and Pathology University of Leuven Leuven Belgium"},{"name":"Oral and Maxillofacial Surgery University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5329-3113","authenticated-orcid":false,"given":"Ana","family":"Castro","sequence":"additional","affiliation":[{"name":"Periodontology &amp; Oral Microbiology Department of Oral Health Sciences University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Coucke","sequence":"additional","affiliation":[{"name":"OMFS IMPATH Research Group Faculty of Medicine Department of Imaging and Pathology University of Leuven Leuven Belgium"},{"name":"Oral and Maxillofacial Surgery University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantinus","family":"Politis","sequence":"additional","affiliation":[{"name":"OMFS IMPATH Research Group Faculty of Medicine Department of Imaging and Pathology University of Leuven Leuven Belgium"},{"name":"Oral and Maxillofacial Surgery University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Verhamme","sequence":"additional","affiliation":[{"name":"Centre for Molecular and Vascular Biology Department of Cardiovascular Sciences University of Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhilde","family":"Jacobs","sequence":"additional","affiliation":[{"name":"OMFS IMPATH Research Group Faculty of Medicine Department of Imaging and Pathology University of Leuven Leuven Belgium"},{"name":"Oral and Maxillofacial Surgery University Hospitals Leuven Leuven Belgium"},{"name":"Department of Dental Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Quirynen","sequence":"additional","affiliation":[{"name":"Periodontology &amp; Oral Microbiology Department of Oral Health Sciences University Hospitals Leuven Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,14]]},"reference":[{"issue":"5","key":"e_1_2_6_2_1","first-page":"64","article-title":"A comprehensive clinical review of Platelet Rich Fibrin (PRF) and its role in promoting tissue healing and regeneration in dentistry. Part 1: Definition, development, biological characteristics and function","volume":"6","author":"Hartshorne J","year":"2016","journal-title":"Int Dent \u2013 African Ed"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12663-012-0351-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/08977190802636713"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.11607\/jomi.7275"},{"issue":"4","key":"e_1_2_6_6_1","first-page":"18","article-title":"Effect of Platelet Rich Fibrin (PRF) on peri\u2010implant soft tissue and crestal bone in one\u2010stage implant placement: A randomized controlled trial","volume":"9","author":"Boora P","year":"2015","journal-title":"J Clin Diagnostic Res"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2008.17.2.28179"},{"issue":"5","key":"e_1_2_6_8_1","first-page":"34","article-title":"A comprehensive clinical review of Platelet Rich Fibrin (PRF) and its role in promoting tissue healing and regeneration in dentistry. Part II: Preparation, optimization, handling and application, benefits and limitation of PRF","volume":"6","author":"Hartshorne J","year":"2016","journal-title":"Int Dent \u2013 African Ed"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12658"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12643"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12612"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-2391(03)00727-4"},{"issue":"5","key":"e_1_2_6_13_1","first-page":"64","article-title":"A comprehensive clinical review of Platelet Rich Fibrin (PRF) and its role in promoting tissue healing and regeneration in dentistry. Part III: Clinical indications of PRF in implant dentistry, periodontology, oral surgery and regenerative","volume":"6","author":"Hartshorne J","year":"2016","journal-title":"Int Dent \u2013 African Ed"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4081\/hr.2015.5844"},{"key":"e_1_2_6_15_1","first-page":"55","article-title":"Une opportunit\u00e9 en paro\u2010implantologie: Le PRF","volume":"42","author":"Choukroun J","year":"2001","journal-title":"Implantodontie"},{"key":"e_1_2_6_16_1","first-page":"305","article-title":"In vitro evaluation of mechanical and structural properties of leukocyte\u2010 platelet\u2010rich fibrin and platelet\u2010rich fibrin matrix","volume":"63","author":"Aggour RL","year":"2018","journal-title":"Egypt Dent J"},{"key":"e_1_2_6_17_1","first-page":"1","article-title":"Characterization of leukocyte\u2010platelet rich fibrin, a novel biomaterial","volume":"103","author":"Madurantakam P","year":"2015","journal-title":"J Vis Exp"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1849207"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8515829"},{"issue":"3433","key":"e_1_2_6_20_1","first-page":"1","article-title":"Biological and mechanical properties of platelet\u2010rich fibrin membranes after thermal manipulation and preparation in a single\u2010syringe closed system","volume":"19","author":"Kardos D","year":"2018","journal-title":"Int J Mol Sci"},{"key":"e_1_2_6_21_1","first-page":"1","article-title":"The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte\u2010 and platelet\u2010rich fibrin (L\u2010PRF) clot and membrane","volume":"29","author":"Ehrenfest DMD","year":"2017","journal-title":"Platelets"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4103\/0972-124X.176390","article-title":"Fibrin network pattern changes of platelet\u2010rich fibrin in young versus old age group of individuals: a cell block cytology study","author":"Yajamanya SR","year":"2016","journal-title":"Indian Soc Periodontol"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2005.07.008"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-012-9349-6"},{"key":"e_1_2_6_25_1","first-page":"405","article-title":"Fibrin formation","volume":"82","author":"Weisel JW","year":"2018","journal-title":"Struct Prop"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ksujds.2013.09.001"},{"key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1111\/j.1749-6632.2001.tb03526.x","article-title":"Role of fibrin matrix in angiogenesis","author":"Hinsbergh VW","year":"2001","journal-title":"Ann New York Acad Sci"},{"key":"e_1_2_6_28_1","first-page":"1","article-title":"Platelet counts in insoluble platelet\u2010rich fibrin clots: a direct method for accurate determination","volume":"6","author":"Kitamura Y","year":"2018","journal-title":"Front Bioeng Biotechol"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjre.12755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jre.12755","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.12755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T20:41:16Z","timestamp":1722890476000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.12755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,14]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1111\/jre.12755"],"URL":"https:\/\/doi.org\/10.1111\/jre.12755","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,14]]},"assertion":[{"value":"2019-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}