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Galectins are glycan\u2010binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galectin\u20108 (Gal\u20108) in the response of gingival connective tissue cells to cyclosporine.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used human gingival fibroblasts and mouse NIH3T3 cells treated with recombinant Gal\u20108 and\/or cyclosporine for analyzing specific mRNA and protein levels through immunoblot, real\u2010time polymerase chain reaction, ELISA and immunofluorescence, pull\u2010down with Gal\u20108\u2010Sepharose for Gal\u20108\u2010to\u2010cell surface glycoprotein interactions, short hairpin RNA for Gal\u20108 silencing and Student's <jats:italic>t<\/jats:italic> test and ANOVA for statistical analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Galectin\u20108 stimulated type I collagen and fibronectin protein levels and potentiated CTGF protein levels in TGF\u2010\u03b21\u2010stimulated human gingival fibroblasts. Gal\u20108 interacted with \u03b15\u03b21\u2010integrin and type II TGF\u2010\u03b2 receptor. Gal\u20108 stimulated fibronectin protein and mRNA levels, and this response was dependent on FAK activity but not Smad2\/3 signaling. Cyclosporine and tumor necrosis factor alpha (TNF\u2010\u03b1) increased Gal\u20108 protein levels. Finally, silencing of galectin\u20108 in NIH3T3 cells abolished cyclosporine\u2010induced fibronectin protein levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken together, these results reveal for the first time Gal\u20108 as a fibrogenic stimulus exerted through \u03b21\u2010integrin\/FAK pathways in human gingival fibroblasts, which can be triggered by cyclosporine. Further studies should explore the involvement of Gal\u20108 in human gingival tissues and its role in drug\u2010induced gingival overgrowth.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12761","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T17:37:15Z","timestamp":1590428235000},"page":"724-733","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Galectin\u20108 mediates fibrogenesis induced by cyclosporine in human gingival fibroblasts"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7314-621X","authenticated-orcid":false,"given":"Patricio C.","family":"Smith","sequence":"first","affiliation":[{"name":"School of Dentistry Faculty of Medicine Pontificia Universidad Cat\u00f3lica de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Metz","sequence":"additional","affiliation":[{"name":"Centro 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